Alexa Fluor® 700 anti-mouse IgG1 Antibody

Pricing & Availability
Clone
RMG1-1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G1
Isotype
Rat IgG
RMG1-1_A700_IgG1_Antibody_100418
Human peripheral blood lymphocytes were stained with (filled histogram) or without (open histogram) purified anti-human CD3 (clone UCHT1, mouse IgG1 isotype) followed by anti-mouse IgG1 (clone RMG1-1) Alexa Fluor® 700.
  • RMG1-1_A700_IgG1_Antibody_100418
    Human peripheral blood lymphocytes were stained with (filled histogram) or without (open histogram) purified anti-human CD3 (clone UCHT1, mouse IgG1 isotype) followed by anti-mouse IgG1 (clone RMG1-1) Alexa Fluor® 700.
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability
406631 25 µg $71.00
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406632 100 µg $188.00
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Description

The RMG1-1 monoclonal antibody reacts with immunoglobulin G1 (IgG1) in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG1-1 monoclonal antibody may be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Rucci F, et al. 2010. P. Natl. Acad. Sci. USA 107:3024. (FC) PubMed
  2. Zhang JG, et al. 2011. Int. Immunopharmacol. 11:1685. PubMed
Product Citations
  1. Long CM, et al. 2021. NPJ Vaccines. 6:38. PubMed
  2. Tesfamariam M, et al. 2022. Microorganisms. 10:. PubMed
RRID
AB_2783323 (BioLegend Cat. No. 406631)
AB_2783323 (BioLegend Cat. No. 406632)
Go To Top Version: 1    Revision Date: 10/05/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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