APC anti-mouse CD127 (IL-7Rα) Antibody

Pricing & Availability
Clone
A7R34 (See other available formats)
Regulatory Status
RUO
Other Names
IL-7 receptor α chain, IL-7Rα
Isotype
Rat IgG2a, κ
A_A7R34_APC_102809
C57BL/6 splenocytes stained with CD3 (145-2C11) PE and A7R34 APC (top) or rat IgG2a, κ APC isotype control (bottom)
  • A_A7R34_APC_102809
    C57BL/6 splenocytes stained with CD3 (145-2C11) PE and A7R34 APC (top) or rat IgG2a, κ APC isotype control (bottom)
  • B_A7R34_APC_102809
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability
135011 25 µg $99.00
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135012 100 µg $325.00
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Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-7Ra-IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Red Laser (633 nm)
Application Notes

A7R34 is able to block clone SB/199 binding to IL-7R.

Application References

(PubMed link indicates BioLegend citation)
  1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
  2. Hashi H, et al. 2001. J. Immunol. 166:3702.
  3. Taylor R, et al. 2007. J. Immunol. 178:5659.
  4. Mazzon C, et al. 2011. Blood. 118:2733. PubMed
  5. Jin J, et al. 2011. J. Immunol. doi:10.4049/jimmunol.1001238. PubMed
Product Citations
  1. Wang X, et al. 2023. Nat Commun. 14:3440. PubMed
  2. Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed
  3. Ali LR, et al. 2023. J Exp Med. 220: . PubMed
  4. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  5. Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed
  6. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  7. Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed
  8. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  9. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  10. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  11. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
  12. Pfenninger P, et al. 2022. Front Immunol. 13:777113. PubMed
  13. Zhao H, et al. 2021. Int J Nanomedicine. 16:4913. PubMed
  14. Nakajima T, et al. 2021. J Exp Med. 218:. PubMed
  15. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  16. Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
  17. Liu W, et al. 2019. Epigenetics. 1.192361111. PubMed
  18. Zaro BW, et al. 2020. eLife. 9:00. PubMed
  19. Xu X, et al. 2018. J Dermatol Sci. 91:134. PubMed
  20. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  21. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  22. Hu Z, et al. 2020. PLoS One. 15:e0228339. PubMed
  23. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  24. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
  25. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  26. Panagi M, et al. 2020. Theranostics. 10:1910. PubMed
  27. Fujimoto M, et al. 2022. Nat Commun. 13:5408. PubMed
  28. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  29. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  30. Ding Z, et al. 2017. Sci Rep. 10.1038/s41598-017-12488-z. PubMed
  31. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
RRID
AB_1937216 (BioLegend Cat. No. 135011)
AB_1937216 (BioLegend Cat. No. 135012)

Antigen Details

Structure
Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Ligand/Receptor
IL-7
Cell Type
B cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246.
3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.

Gene ID
16197 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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