APC anti-mouse CD135 Antibody

Pricing & Availability
Clone
A2F10 (See other available formats)
Regulatory Status
RUO
Other Names
Flk-2, Flt3, Ly-72
Isotype
Rat IgG2a, κ
A_A2F10_APC_121509
C57BL/6 bone marrow cells stained with A2F10 APC and CD45R/B220 (RA3-6B2) plus CD11b (M1/70) PE
  • A_A2F10_APC_121509
    C57BL/6 bone marrow cells stained with A2F10 APC and CD45R/B220 (RA3-6B2) plus CD11b (M1/70) PE
  • B_A2F10_121509
    C57BL/6 bone marrow cells stained with rat IgG2a APC isotype control and and CD45R/B220 (RA3-6B2) plus CD11b (M1/70) PE
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Cat # Size Price Quantity Check Availability
135309 25 µg $95.00
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135310 100 µg $267.00
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Description

CD135, also known as Flk-2, Flt3, and Ly-72, is a type III tyrosine kinase receptor. It is expressed on early B lymphoid lineage cells in bone marrow, on primitive myeloid progenitors within the BM CD34+ cell population. Ligation of Flk-2 with Flt3 ligand regulates the growth of hematopoietic stem cells and promotes the survival of primitive hematopoietic progenitor cells with myeloid as well as B lymphoid potential. It was reported that the receptor tyrosine kinase Flt3 is required for dendritic cell development. Combined signaling through interleukin-7 receptors and Flt3 selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Flt3 transfected cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Sergejeva S, et al. 2004. Blood 103:1270.
  2. Auffray C, et al. 2009. J. Exp. Med. 206:595.
  3. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
Product Citations
  1. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  2. Song R, et al. 2022. Immunology. 165:274. PubMed
  3. Tu J, et al. 2023. Ann Rheum Dis. 82:198. PubMed
  4. Zhu J, et al. 2022. Nat Commun. 13:7466. PubMed
  5. Jabbar S, et al. 2022. Exp Hematol Oncol. 11:83. PubMed
  6. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  7. Han X, et al. 2023. J Clin Invest. 133:. PubMed
  8. van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed
  9. Mitroulis I et al. 2018. Cell. 172(1-2):147-161 . PubMed
  10. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  11. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  12. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  13. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  14. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  15. Schlager S, et al. 2015. J Leukoc Biol. 98: 837 - 850. PubMed
  16. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  17. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
  18. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  19. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  20. Gao S, et al. 2022. Cell Res. 32:38. PubMed
  21. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  22. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  23. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  24. Jonas B, et al. 2016. PLoS One. 11: 0159189. PubMed
  25. Wuggenig P, et al. 2020. Commun Biol. 3:130. PubMed
  26. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  27. Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
  28. Oppezzo A, et al. 2020. J Clin Invest. 130:1377. PubMed
  29. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  30. Li Z, et al. 2017. Sci Rep. 7:40380. PubMed
  31. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
  32. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  33. Giridhar P, et al. 2016. J Immunol. 197(11):4228-4239. PubMed
RRID
AB_1953264 (BioLegend Cat. No. 135309)
AB_1953264 (BioLegend Cat. No. 135310)

Antigen Details

Structure
A 135-150 kD molecule belonging to the tyrosine kinase receptor family.
Distribution

Expressed on early B lymphoid lineage cells in juvenile and adult bone marrow, primitive myeloid progenitors within the BM CD34+ cell population.

Function
Regulate the growth of hematopoietic stem cells and promote the survival of primitive hematopoietic progenitor cells.
Ligand/Receptor
Flk-2/FLT3 ligand
Cell Type
B cells, Hematopoietic stem and progenitors
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Waskow C, et al.Nat. Immunol. 9:676
2. Veiby OP, et al. 1996. Blood 88(4):1256
3. Veiby OP, et al. 1996. J. Immunol. 157(7):2953
4. Mattews W, et al. 1991. Cell. 65(7):1143
5. Hannum C, et al. 1994. Nature 368(2):643
6. Ogawa M, et al. 1998. Exp Hematol. 26(6):478

Gene ID
14255 View all products for this Gene ID
UniProt
View information about CD135 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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