Brilliant Violet 421™ anti-mouse CD135 Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse Flt3 transfected cell line

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.

Concentration

µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of the this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application References

(PubMed link indicates BioLegend citation)

1. Sergejeva S, et al. 2004. Blood 103:1270.
2. Auffray C, et al. 2009. J. Exp. Med. 206:595.
3. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed

Product Citations

1. Zhou L, et al. 2022. PLoS Negl Trop Dis. 16:e0010851. PubMed
2. Liu X, et al. 2022. Nutrients. 14: . PubMed
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5. Li S, et al. 2022. Elife. 11:. PubMed
6. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
7. Zhou W, et al. 2019. Cell Syst. 0.597916667. PubMed
8. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
9. Bonnet C, et al. 2021. iScience. 24:103399. PubMed
10. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
11. Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
12. Bennion BG, et al. 2020. Cell Reports. 31(11):107771. PubMed
13. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
14. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
15. Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
16. Bellelli R et al. 2018. Molecular cell. 70(4):707-721 . PubMed
17. Montecino-Rodriguez E, et al. 2020. STAR Protoc. 1:100159. PubMed
18. Oka Y, et al. 2020. Sci Adv. 6:. PubMed
19. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
20. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
21. Zhang J, et al. 2021. Stem Cell Res Ther. 12:579. PubMed
22. Young K, et al. 2021. Cell Stem Cell. . PubMed

RRID

AB_2562338 (BioLegend Cat. No. 135313)
AB_2562338 (BioLegend Cat. No. 135315)
AB_2562338 (BioLegend Cat. No. 135314)

Antigen Details

Structure

A 135-150 kD molecule belonging to the tyrosine kinase receptor family.

Distribution

Expressed on early B lymphoid lineage cells in juvenile and adult bone marrow, primitive myeloid progenitors within the BM CD34+ cell population.

Function

Regulate the growth of hematopoietic stem cells and promote the survival of primitive hematopoietic progenitor cells.

Ligand/Receptor

Flk-2/FLT3 ligand

Cell Type

B cells, Hematopoietic stem and progenitors

Biology Area

Immunology

Molecular Family

CD Molecules

Antigen References

1. Waskow C, et al.Nat. Immunol. 9:676
2. Veiby OP, et al. 1996. Blood 88(4):1256
3. Veiby OP, et al. 1996. J. Immunol. 157(7):2953
4. Mattews W, et al. 1991. Cell. 65(7):1143
5. Hannum C, et al. 1994. Nature 368(2):643
6. Ogawa M, et al. 1998. Exp Hematol. 26(6):478

Gene ID

14255 View all products for this Gene ID

UniProt

View information about CD135 on UniProt.org

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Other Formats