PE/Cyanine5 anti-mouse CD135 Antibody

Pricing & Availability
Clone
A2F10 (See other available formats)
Regulatory Status
RUO
Other Names
Flk-2, Flt3, Ly-72
Isotype
Rat IgG2a, κ
A2F10_PECyanine5_CD135_Antibody_073124
C57BL/6 mouse bone marrow cells were stained with anti-mouse CD45R/B220 (clone RA3-6B2) FITC and anti-mouse CD135 (clone A2F10) PE/Cyanine5 (left) or rat IgG2a, κ PE/Cyanine5 isotype control (right).
  • A2F10_PECyanine5_CD135_Antibody_073124
    C57BL/6 mouse bone marrow cells were stained with anti-mouse CD45R/B220 (clone RA3-6B2) FITC and anti-mouse CD135 (clone A2F10) PE/Cyanine5 (left) or rat IgG2a, κ PE/Cyanine5 isotype control (right).
Compare all formats See PE/Cyanine5 spectral data
Cat # Size Price Quantity Check Availability
135311 25 µg $106.00
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135312 100 µg $340.00
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Description

CD135, also known as Flk-2, Flt3, and Ly-72, is a type III tyrosine kinase receptor. It is expressed on early B lymphoid lineage cells in bone marrow, on primitive myeloid progenitors within the BM CD34+ cell population. Ligation of Flk-2 with Flt3 ligand regulates the growth of hematopoietic stem cells and promotes the survival of primitive hematopoietic progenitor cells with myeloid as well as B lymphoid potential. It was reported that the receptor tyrosine kinase Flt3 is required for dendritic cell development. Combined signaling through interleukin-7 receptors and Flt3 selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Flt3 transfected cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Sergejeva S, et al. 2004. Blood 103:1270.
  2. Auffray C, et al. 2009. J. Exp. Med. 206:595.
  3. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
Product Citations
  1. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  2. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  3. Meng Y, et al. 2023. Cell Mol Biol Lett. 28:45. PubMed
  4. Lu X, et al. 2016. Nat Commun. 7: 12719. PubMed
  5. Bahal R, et al. 2016. Nat Commun. 7:13304. PubMed
  6. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  7. Gao Y, et al. 2020. Immunity. 52(6):1007-1021.e8. PubMed
  8. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  9. Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed
  10. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
  11. Das A, et al. 2020. J Bone Miner Res. 36:199. PubMed
RRID
AB_2107049 (BioLegend Cat. No. 135311)
AB_2107049 (BioLegend Cat. No. 135312)

Antigen Details

Structure
A 135-150 kD molecule belonging to the tyrosine kinase receptor family.
Distribution

Expressed on early B lymphoid lineage cells in juvenile and adult bone marrow, primitive myeloid progenitors within the BM CD34+ cell population.

Function
Regulate the growth of hematopoietic stem cells and promote the survival of primitive hematopoietic progenitor cells.
Ligand/Receptor
Flk-2/FLT3 ligand
Cell Type
B cells, Hematopoietic stem and progenitors
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Waskow C, et al.Nat. Immunol. 9:676
2. Veiby OP, et al. 1996. Blood 88(4):1256
3. Veiby OP, et al. 1996. J. Immunol. 157(7):2953
4. Mattews W, et al. 1991. Cell. 65(7):1143
5. Hannum C, et al. 1994. Nature 368(2):643
6. Ogawa M, et al. 1998. Exp Hematol. 26(6):478

Gene ID
14255 View all products for this Gene ID
UniProt
View information about CD135 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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