APC/Cyanine7 anti-mouse IgD Antibody

Pricing & Availability
Clone
11-26c.2a (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin D
Isotype
Rat IgG2a, κ
11-26c.2a_APCCy7_092110
C57BL/6 splenocytes stained with 11-26c.2a APC/Cyanine7
  • 11-26c.2a_APCCy7_092110
    C57BL/6 splenocytes stained with 11-26c.2a APC/Cyanine7
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability
405715 25 µg $129.00
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405716 100 µg $323.00
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Description

Surface IgD is an important B cell differentiation marker.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.

Application References

(PubMed link indicates BioLegend citation)
  1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
  2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
  3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
  4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
  5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
  7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
  8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
  9. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Hu Q, et al. 2022. Cell Rep. 40:111035. PubMed
  2. Tas JMJ, et al. 2022. Immunity. 55:1856. PubMed
  3. Ke F, et al. 2023. Elife. 12:. PubMed
  4. Ludwig J, et al. 2023. NPJ Vaccines. 8:52. PubMed
  5. Turner J, Benet Z, and Grigorova I. 2017. J Immunol. 10.4049/jimmunol.1700267. PubMed
  6. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  7. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  8. Suah AN, et al. 2021. J Clin Invest. 131:. PubMed
  9. Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
  10. Proudhon C, et al. 2016. Cell Rep. 15:2159-2169. PubMed
  11. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  12. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
  13. Geng T, et al. 2022. Methods Mol Biol. 2585:71. PubMed
  14. Prados A, et al. 2021. Nat Immunol. 22:510. PubMed
  15. Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed
  16. Reynolds A, et al. 2015. J Immunol. 194:231. PubMed
  17. Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed
  18. Wilmore JR et al. 2018. Cell host & microbe. 23(3):302-311 . PubMed
  19. Webster P, et al. 2018. Nat Commun. 9:2649. PubMed
  20. Saumyaa S, et al. 2013. Infect Immun . 81:3426. PubMed
  21. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  22. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  23. Nanou A, et al. 2021. Cell Reports. 35(8):109168. PubMed
  24. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  25. Xiao G, et al. 2018. Cell. 173:470. PubMed
  26. Scally S, et al. 2017. Nat Commun. . 10.1038/s41467-017-01924-3. PubMed
  27. Shehata L, et al. 2019. Cell Rep. 28:3300. PubMed
  28. Zhang H, et al. 2021. Cell Reports. 35(6):109096. PubMed
  29. Wilmore JR, et al. 2022. Front Immunol. 12:791095. PubMed
  30. Burnett DL, et al. 2021. Immunity. S1074-7613:00457. PubMed
  31. Lazarian G, et al. 2021. Cancer Cell. 39(3):380-393.e8. PubMed
  32. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  33. Palmer V, et al. 2015. J Immunol. 195: 1524-1537. PubMed
  34. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  35. Schardt JS, et al. 2021. Sci Rep. 11:20738. PubMed
  36. Jones D, et al. 2015. J Immunol. 195: 4753 - 4759. PubMed
  37. Niemeyer BF, et al. 2021. PLoS Pathog. 17:e1010019. PubMed
  38. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  39. Laczkó D, et al. 2020. Immunity. 53:724. PubMed
RRID
AB_10662544 (BioLegend Cat. No. 405715)
AB_10662544 (BioLegend Cat. No. 405716)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation
Cell Type
B cells
Biology Area
Immunology
Gene ID
380797 View all products for this Gene ID
UniProt
View information about IgD on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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