APC Goat anti-rat IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4054 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal IgG
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Cat # Size Price Quantity Check Availability
405407 100 µg $237.00
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Description

Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.

Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

This goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.

Application References

(PubMed link indicates BioLegend citation)
  1. Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed
  2. Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed
  3. Baba T, et al. 2020. eLife. 9:e54257. (IHC-P)
Product Citations
  1. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  2. Kosti P, et al. 2021. Cell Reports Medicine. 2(4):100227. PubMed
  3. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
  4. Tokarev A, et al. 2011. J Virol. 85:51:00. PubMed
  5. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  6. Zornetzer G, et al. 2010. J Virol. 84:11297. PubMed
  7. Chang DY, et al. 2021. Front Pharmacol. 12:729334. PubMed
  8. Drokhlyansky E, et al. 2017. Proc Natl Acad Sci U S A. 114(1):E95-E104. PubMed
  9. Maeda Y, et al. 2015. J Virol. 89:502. PubMed
RRID
AB_315018 (BioLegend Cat. No. 405407)
Go To Top Version: 2    Revision Date: 02/08/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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