Biotin anti-mouse CD127 (IL-7Rα) Antibody

Pricing & Availability
Clone
A7R34 (See other available formats)
Regulatory Status
RUO
Other Names
IL-7 receptor α chain, IL-7Rα
Isotype
Rat IgG2a, κ
A_A7R34_Biotin_120309
C57BL/6 mouse splenocytes stained with CD3ε (145-2C11) APC and A7R34 biotin (top) or rIgG2a, κ biotin isotype control (bottom), then developed with Sav-PE.
  • A_A7R34_Biotin_120309
    C57BL/6 mouse splenocytes stained with CD3ε (145-2C11) APC and A7R34 biotin (top) or rIgG2a, κ biotin isotype control (bottom), then developed with Sav-PE.
  • B_A7R34_Biotin_120309
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135005 50 µg $77.00
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135006 500 µg $314.00
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Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-7Ra-IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

A7R34 is able to block clone SB/199 binding to IL-7R.

Application References

(PubMed link indicates BioLegend citation)
  1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
  2. Hashi H, et al. 2001. J. Immunol. 166:3702.
  3. Taylor R, et al. 2007. J. Immunol. 178:5659.
  4. Mazzon C, et al. 2011. Blood. 118:2733. PubMed
  5. Jin J, et al. 2011. J. Immunol. doi:10.4049/jimmunol.1001238. PubMed
Product Citations
  1. Florentin J, et al. 2022. Front Immunol. 13:882484. PubMed
  2. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  3. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  4. Lu SX, et al. 2021. Cell. . PubMed
  5. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  6. Dave K et al. 2017. eLife. 6 pii: e23382. PubMed
  7. Liu YY, et al. 2020. Chin Med J (Engl). 133:1688. PubMed
  8. Kim SP, et al. 2021. Cell Reports. 36(9):109626. PubMed
  9. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  10. Knolle MD et al. 2018. Frontiers in immunology. 1.925 . PubMed
  11. Yankova E, et al. 2021. Nature. 593:597. PubMed
  12. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  13. Bouchareychas L, et al. 2020. Cell Reports. 32(2):107881. PubMed
  14. Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed
  15. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  16. Michel A, et al. 2013. J Immunol. 90:5534. PubMed
  17. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
  18. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  19. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  20. Wang J, et al. 2020. Cell Stem Cell. 27(1):81-97. PubMed
  21. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
RRID
AB_2126118 (BioLegend Cat. No. 135005)
AB_2126118 (BioLegend Cat. No. 135006)

Antigen Details

Structure
Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Ligand/Receptor
IL-7
Cell Type
B cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246.
3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.

Gene ID
16197 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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