BL/6 mouse lymph nodes, fixed O/N in PLP, blocked with 10% rat serum, stained with CD11b-BV421™ (red), B220-Alexa Fluor® 647 (blue), CD14-FITC (green) in 1% BSA and 0.1% Tween-20 in PBS. Images were acquired with an automated widefield microscope (Nikon Eclipse Ti) and a CCD camera (QImaging Retiga 2000R). Emitted light was collected through 440/40, 525/50, and 700/75 nm bandpass filters. Images provided by Ann Haberman and Christine Podolski, Yale University.
BL/6 mouse lymph nodes, fixed O/N in PLP, blocked with 10% rat serum, stained with CD11b-BV421™ (red), B220-Alexa Fluor® 647 (blue), CD14-FITC (green) in 1% BSA and 0.1% Tween-20 in PBS. Images were acquired with an automated widefield microscope (Nikon Eclipse Ti) and a CCD camera (QImaging Retiga 2000R). Emitted light was collected through 440/40, 525/50, and 700/75 nm bandpass filters. Images provided by Ann Haberman and Christine Podolski, Yale University.
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 421™ (filled histogram) or rat IgG2b, κ Brilliant Violet 421™ isotype control (open histogram). Data shown was gated on myeloid cell population.
CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For flow cytometric staining using the µL sizes, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes
Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).
Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, immunohistochemistry of acetone-fixed frozen sections5,11-13, and spatial biology (IBEX)35,36. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).
Application References
(PubMed link indicates BioLegend citation)
Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)
Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)
Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)
Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)
Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)
Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)
D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)
Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)
Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)
Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)
Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)
Olver S, et al. 2006. Cancer Research 66:571. (FC)
Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed
Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)
Dzhagalov I, et al. 2007. Blood 109:1620. (FC)
Fazilleau N, et al. 2007. Nature Immunol. 8:753.
Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed
Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed
Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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