Brilliant Violet 650™ anti-mouse/human CD11b Antibody

Pricing & Availability
Clone
M1/70 (See other available formats)
Regulatory Status
RUO
Other Names
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Isotype
Rat IgG2b, κ
M1slash70_BV650_020212
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 650™. Data shown was gated on myeloid cell population.
  • M1slash70_BV650_020212
    C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 650™. Data shown was gated on myeloid cell population.
Compare all formats See Brilliant Violet 650™ spectral data
Cat # Size Price Quantity Check Availability
101239 125 µL $209.00
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101259 50 µg $273.00
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Description

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Technical data sheet

Product Details

Verified Reactivity
Mouse, Human, Cynomolgus, Rhesus
Reported Reactivity
Chimpanzee, Baboon, Rabbit
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.3 µg per million cells in 100 µl volume. For flow cytometric staining using the test size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).

Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, immunohistochemistry of acetone-fixed frozen sections5,11-13, and spatial biology (IBEX)35,36. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).

Application References

(PubMed link indicates BioLegend citation)
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)
  2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)
  3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)
  4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)
  5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
  6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)
  7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)
  8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)
  9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)
  10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)
  11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
  13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)
  14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)
  15. Olver S, et al. 2006. Cancer Research 66:571. (FC)
  16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed
  17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)
  18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC)
  19. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed
  21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed
  22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed
  23. Kim DD, et al. 2008. Blood 112:1109. PubMed
  24. Guo Y, et al. 2008. Blood 112:480. PubMed
  25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed
  26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed
  27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  29. Weber GF, et al. 2014. J Exp Med. 211:1243. PubMed
  30. Ashok A, et al. 2015. Toxicol Sci. 143:64. PubMed
  31. Price PJ, et al. 2015. J Immunol. 194:1164. PubMed
  32. Doni A, et al. 2015. J Exp Med. 212:905. PubMed
  33. Ferreira R, et al. 2016. J Infect Dis. 213: 669 - 673. PubMed
  34. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  35. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  36. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Cautivo KM, et al. 2022. Immunity. 55:254. PubMed
  2. Liu H, et al. 2022. Cancer Immunol Res. 10:1028. PubMed
  3. Brigo N, et al. 2022. Bio Protoc. 12:e4440. PubMed
  4. Sieow JL, et al. 2023. Int J Mol Sci. 24: . PubMed
  5. Kang J, et al. 2023. JCI Insight. 8: . PubMed
  6. Özcan A, et al. 2020. J Invest Dermatol. 1003:140. PubMed
  7. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  8. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  9. Sierro F, et al. 2017. Immunity. 47:374. PubMed
  10. Bajaj R, et al. 2022. Cell Rep. 40:111429. PubMed
  11. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  12. Brigo N, et al. 2023. Cells. 12:. PubMed
  13. Liu J, et al. 2023. Nat Commun. 14:2806. PubMed
  14. Zengel J, et al. 2023. Nat Methods. . PubMed
  15. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  16. Hulsebus HJ, et al. 2022. Front Immunol. 13:884719. PubMed
  17. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  18. Grubman A, et al. 2020. Stem Cell Reports. 1.290277778. PubMed
  19. Matsushita Y, et al. 2021. J Bone Miner Res. 36:1145. PubMed
  20. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  21. Gimblet C et al. 2017. Cell host & microbe. 22(1):13-24 . PubMed
  22. Hagan T, et al. 2020. Cell. 178(6):1313-1328.e13.. PubMed
  23. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  24. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  25. Brestoff JR, et al. 2021. Cell Metab. 33:270. PubMed
  26. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  27. Demircioglu F, et al. 2020. Nat Commun. 11:1290. PubMed
  28. Lees JG, et al. 2020. PLoS One. 15:e0238164. PubMed
  29. Bagayoko S, et al. 2021. PLoS Pathog. 17:e1009927. PubMed
  30. Tuit S, et al. 2019. Cell Rep. 29:1221. PubMed
  31. Li H, et al. 2019. Cell. 176:775. PubMed
  32. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  33. Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed
  34. Thion MS et al. 2018. Cell. 172(3):500-516 . PubMed
  35. Bittner–Eddy PD, et al. 2017. Front Immunol. 1.304166667. PubMed
  36. Uceda-Castro R, et al. 2022. Cell Rep Med. 3:100821. PubMed
  37. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  38. Benechet AP, et al. 2019. Nature. 574:200. PubMed
  39. Costa A et al. 2018. Cancer cell. 33(3):463-479 . PubMed
  40. Warheit-Niemi HI, et al. 2022. JCI Insight. 7:. PubMed
  41. Taniguchi H, et al. 2022. Cell Rep. 39:110814. PubMed
  42. Grigoryan L, et al. 2022. NPJ Vaccines. 7:55. PubMed
  43. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  44. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  45. Marn Franco JL, et al. 2020. Cell Reports. 33(13):108547. PubMed
  46. BenYoucef A, et al. 2020. J Allergy Clin Immunol. 145:1690. PubMed
  47. Gniadek TJ, et al. 2020. J Immunother. 43:217. PubMed
  48. Tan X, et al. 2021. Sci Adv. 7: . PubMed
  49. De Simone G, et al. 2021. Immunity. :. PubMed
  50. Tritz ZP, et al. 2020. J Immunol. 205:1228. PubMed
  51. Li Y, et al. 2020. Cell Reports. 27(10):2809-2816.e3.. PubMed
  52. Chen YG, et al. 2020. Molecular Cell. 76(1):96-109. PubMed
  53. Lin J, et al. 2021. Nat Commun. 12:5321. PubMed
  54. Blriot C, et al. 2021. Immunity. :. PubMed
  55. Shreeve N, et al. 2021. Immunity. 54(6):1231-1244.e4. PubMed
  56. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  57. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  58. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  59. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  60. Colliou N, et al. 2015. Toxins. 7: 3805 - 3817. PubMed
  61. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  62. Su W et al. 2019. Cancer Cell. 36(2):139-155 . PubMed
  63. Blagih J, et al. 2020. Cell Rep. 30:481. PubMed
  64. Abdelfattah N, et al. 2022. Nat Commun. 13:767. PubMed
  65. Riedel R, et al. 2020. Nat Commun. 11:2570. PubMed
  66. Rosenbaum SR, et al. 2020. Cell Rep. 30:510. PubMed
  67. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  68. Shen XR, et al. 2021. Viruses. 13:. PubMed
  69. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  70. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  71. Teo TH, et al. 2018. Nat Commun. 9:3905. PubMed
  72. Maniati E, et al. 2020. Cell Rep. 30:525. PubMed
  73. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
  74. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
  75. Moreno M et al. 2014. The Journal of Neuroscience. 34(24):8175-85 . PubMed
  76. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  77. Lee A, et al. 2022. Nat Commun. 13:549. PubMed
  78. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  79. Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed
  80. O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed
  81. Liu Z, et al. 2020. STAR Protoc. 1:100029. PubMed
  82. Terlizzi M, et al. 2021. Cell Physiol Biochem. 55:539. PubMed
  83. Andreata F, et al. 2021. STAR Protoc. 2:100831. PubMed
  84. Yu YR, et al. 2020. STAR Protocols. 11(3):e0150606. PubMed
RRID
AB_11125575 (BioLegend Cat. No. 101239)
AB_11125575 (BioLegend Cat. No. 101259)

Antigen Details

Structure
Integrin family, associates with integrin β2 (CD18), 170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells

Function
Adhesion, chemotaxis
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Coxon A, et al. 1996. Immunity 5:653.

Gene ID
16409 View all products for this Gene ID 3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org

Other Formats

View All CD11b Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse/human CD11b M1/70 FC
Biotin anti-mouse/human CD11b M1/70 FC
FITC anti-mouse/human CD11b M1/70 FC
PE anti-mouse/human CD11b M1/70 FC,IHC
PE/Cyanine5 anti-mouse/human CD11b M1/70 FC
Purified anti-mouse/human CD11b M1/70 FC,CyTOF®,IP,IHC-F,ICC
PE/Cyanine7 anti-mouse/human CD11b M1/70 FC
Alexa Fluor® 488 anti-mouse/human CD11b M1/70 FC,IHC-F,3D IHC,SB
Alexa Fluor® 647 anti-mouse/human CD11b M1/70 FC,3D IHC,SB
Alexa Fluor® 700 anti-mouse/human CD11b M1/70 FC
Pacific Blue™ anti-mouse/human CD11b M1/70 FC
APC/Cyanine7 anti-mouse/human CD11b M1/70 FC
PerCP/Cyanine5.5 anti-mouse/human CD11b M1/70 FC
PerCP anti-mouse/human CD11b M1/70 FC
Brilliant Violet 421™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 570™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 605™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 650™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 711™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 785™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 510™ anti-mouse/human CD11b M1/70 FC,ICC
Ultra-LEAF™ Purified anti-mouse/human CD11b M1/70 FC,CyTOF®,IP,Block,Depletion,IHC-F,ICC
Purified anti-mouse/human CD11b (Maxpar® Ready) M1/70 FC,CyTOF®
Alexa Fluor® 594 anti-mouse/human CD11b M1/70 IHC-F,FC
PE/Dazzle™ 594 anti-mouse/human CD11b M1/70 FC
APC/Fire™ 750 anti-mouse/human CD11b M1/70 FC
TotalSeq™-A0014 anti-mouse/human CD11b M1/70 PG
Brilliant Violet 750™ anti-mouse/human CD11b M1/70 FC
TotalSeq™-B0014 anti-mouse/human CD11b M1/70 PG
TotalSeq™-C0014 anti-mouse/human CD11b M1/70 PG
Spark NIR™ 685 anti-mouse/human CD11b M1/70 FC
PE/Fire™ 640 anti-mouse/human CD11b M1/70 FC
Spark YG™ 593 anti-mouse/human CD11b M1/70 FC
Spark YG™ 570 anti-mouse/human CD11b M1/70 IHC-F
PE/Fire™ 810 anti-mouse/human CD11b M1/70 FC
APC/Fire™ 810 anti-mouse/human CD11b Antibody M1/70 FC
Spark Blue™ 550 anti-mouse/human CD11b M1/70 FC
Spark UV™ 387 anti-mouse/human CD11b M1/70 FC
PerCP/Fire™ 806 anti-mouse/human CD11b M1/70 FC
PerCP/Fire™ 780 anti-mouse/human CD11b M1/70 FC
Spark Blue™ 574 anti-mouse/human CD11b (Flexi-Fluor™) M1/70 FC
KIRAVIA Blue 520™ anti-mouse/human CD11b M1/70 FC
PE/Fire™ 744 anti-mouse/human CD11b M1/70 FC
Spark PLUS UV395™ anti-mouse/human CD11b M1/70 FC
Spark Red™ 718 anti-mouse/human CD11b (Flexi-Fluor™) M1/70 FC
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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