Brilliant Violet 510™ anti-human CD56 (NCAM) Antibody

Pricing & Availability
Clone
HCD56 (See other available formats)
Regulatory Status
RUO
Other Names
Leu-19, NKH1
Isotype
Mouse IgG1, κ
HCD56_BV510_CD56_Antibody_FC_082512
Human peripheral blood lymphocytes were stained with CD3 APC and CD56 (clone HCD56) Brilliant Violet 510™.
  • HCD56_BV510_CD56_Antibody_FC_082512
    Human peripheral blood lymphocytes were stained with CD3 APC and CD56 (clone HCD56) Brilliant Violet 510™.
Compare all formats See Brilliant Violet 510™ spectral data
Cat # Size Price Quantity Check Availability
318339 25 tests $215.00
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318340 100 tests $407.00
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Description

CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone HCD56 is not recommended for immunohistochemistry formalin-fixed paraffin-embedded tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations
  1. Lima NS, et al. 2022. Nat Commun. 13:7733. PubMed
  2. Townsley SM, et al. 2021. Cell Host Microbe. 29(4):564-578.e9. PubMed
  3. Wang H, et al. 2022. Front Immunol. 13:852436. PubMed
  4. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  5. Wang H, et al. 2022. iScience. 25:105065. PubMed
  6. Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed
  7. Rölle A, et al. 2018. Cell Rep. 24:1967. PubMed
  8. Jiang Y, et al. 2020. Clin Immunol. 218:108516. PubMed
  9. Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed
  10. Tan J, et al. 2021. Sci Transl Med. 13: . PubMed
  11. Serra EG, et al. 2020. Nat Commun. 11:995. PubMed
  12. Abd Hamid M, et al. 2020. Cancer Immunology Research. 8(2):203-216. PubMed
  13. Nel I, et al. 2021. Diabetologia. 64(10):2306-2321. PubMed
  14. Maas RJ, et al. 2020. Oncoimmunology. 9:1843247. PubMed
  15. Port JR, et al. 2020. J Virol. 94:. PubMed
  16. Cohen CA, et al. 2021. Nat Commun. 12:4678. PubMed
  17. Townsley SM, et al. 2021. STAR Protocols. 2(3):100771. PubMed
  18. Wang L, et al. 2021. Science. 373:. PubMed
  19. Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
  20. Dacon C, et al. 2022. Cell Host Microbe. Online ahead of print. PubMed
  21. Cho H, et al. 2021. Sci Transl Med. 13:eabj5413. PubMed
  22. Yang Zhou J, et al. 2022. Front Immunol. 13:898473. PubMed
  23. Van der Meer JMR, et al. 2021. Oncoimmunology. 10:1981049. PubMed
  24. Van der Meer JM, et al. 2020. Cancer Immunol Immunother. . PubMed
  25. Abd Hamid M et al. 2019. Cancer Immunol Res. 7(8):1293-1306 . PubMed
RRID
AB_2561385 (BioLegend Cat. No. 318339)
AB_2561385 (BioLegend Cat. No. 318340)

Antigen Details

Structure
Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution

NK cells, T subset, neural tissue, some LGL and myeloid leukemias

Function
Adhesion
Ligand/Receptor
Heparin sulfate
Cell Type
B cells, Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Lanier L, et al. 1991. J. Immunol. 146:4421.
2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71.
3. Cremer H, et al. 1994. Nature 367:455.

Gene ID
4684 View all products for this Gene ID
UniProt
View information about CD56 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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