Brilliant Violet 510™ anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
1A8_BV510_Ly6G_Antibody_FC_120213
C57BL/6 mouse bone marrow cells were stained with Ly-6G (clone 1A8) Brilliant Violet 510™ (filled histogram) or rat IgG2a, κ Brilliant Violet 510™ isotype control (open histogram). Data shown was gated on myeloid cell population.
  • 1A8_BV510_Ly6G_Antibody_FC_120213
    C57BL/6 mouse bone marrow cells were stained with Ly-6G (clone 1A8) Brilliant Violet 510™ (filled histogram) or rat IgG2a, κ Brilliant Violet 510™ isotype control (open histogram). Data shown was gated on myeloid cell population.
Compare all formats See Brilliant Violet 510™ spectral data
Cat # Size Price Quantity Check Availability
127633 50 µg $297.00
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
  20. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  21. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed
  2. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
  3. Wanrooy BJ, et al. 2022. Immunol Cell Biol. 100:482. PubMed
  4. Drummer C, et al. 2023. Front Immunol. 14:1113883. PubMed
  5. Friedman D, et al. 2022. J Immunol. 208:1845. PubMed
  6. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  7. Takimoto Y, et al. 2023. iScience. 26:106220. PubMed
  8. Barberio AE, et al. 2023. Bioeng Transl Med. 8:e10453. PubMed
  9. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  10. Petenkova A, et al. 2023. Nat Commun. 14:2761. PubMed
  11. Postat J et al. 2018. Immunity. 49(4):654-665 . PubMed
  12. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  13. Barberio AE, et al. 2020. ACS Nano. 14:11238. PubMed
  14. Bertocchi A, et al. 2021. Cancer Cell. 39(5):708-724.e11. PubMed
  15. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  16. Amor C, et al. 2020. Nature. 583:127. PubMed
  17. Ratitong B, et al. 2021. Cell Reports. 35(7):109139. PubMed
  18. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  19. Eisele AS, et al. 2022. Elife. 11:. PubMed
  20. Cassidy BR, et al. 2020. J Neuroinflammation. 17:259. PubMed
  21. Guérin MV, et al. 2019. Nat Commun. 10:4131. PubMed
  22. Salvioni A et al. 2019. Cell Rep. 27(11):3254-3268 . PubMed
  23. Fennell LM, et al. 2020. EMBO J. 39:e103303. PubMed
  24. Lu X, et al. 2020. Sci Transl Med. 12:. PubMed
  25. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  26. Ding Y, et al. 2022. Cell Death Dis. 13:996. PubMed
  27. Kamp M, et al. 2016. PLoS One. 11: 0163750. PubMed
  28. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  29. Uribesalgo I, et al. 2019. EMBO Mol Med. 11:e9266. PubMed
  30. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  31. Monaghan KL, et al. 2020. J Vis Exp. . PubMed
  32. Coronel MM, et al. 2020. Sci Adv. 6:eaba5573. PubMed
  33. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  34. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  35. Sung PS, et al. 2022. JCI Insight. :. PubMed
  36. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  37. Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
  38. Lee HN, et al. 2022. JCI Insight. 7:. PubMed
  39. Teijeira á, et al. 2020. Immunity. 52(5):856-871. PubMed
  40. Jones GS, et al. 2020. mSphere. 5:. PubMed
  41. Kilgore AM, et al. 2020. J Immunol. 204:510. PubMed
  42. Goldberg MF et al. 2018. Immunity. 49(6):1090-1102 . PubMed
RRID
AB_2562937 (BioLegend Cat. No. 127633)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Other Formats

View All Ly-6G Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse Ly-6G 1A8 IHC-F,SB
Purified anti-mouse Ly-6G 1A8 FC,IHC-F,IHC-P,SB
Biotin anti-mouse Ly-6G 1A8 FC,IHC
FITC anti-mouse Ly-6G 1A8 FC
PE anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 647 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Pacific Blue™ anti-mouse Ly-6G 1A8 FC
APC anti-mouse Ly-6G 1A8 FC
PerCP/Cyanine5.5 anti-mouse Ly-6G 1A8 FC
PE/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 700 anti-mouse Ly-6G 1A8 FC,IHC
APC/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 488 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Brilliant Violet 421™ anti-mouse Ly-6G 1A8 FC,IHC-F
Brilliant Violet 570™ anti-mouse Ly-6G 1A8 FC
Ultra-LEAF™ Purified anti-mouse Ly-6G 1A8 FC,Depletion,IHC
Brilliant Violet 510™ anti-mouse Ly-6G 1A8 FC
Purified anti-mouse Ly-6G (Maxpar® Ready) 1A8 FC,CyTOF®,WB
Brilliant Violet 650™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 711™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 605™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 785™ anti-mouse Ly-6G 1A8 FC
PE/Dazzle™ 594 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 750 anti-mouse Ly-6G 1A8 FC
PerCP anti-mouse Ly-6G 1A8 FC
TotalSeq™-A0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-C0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-B0015 anti-mouse Ly-6G 1A8 PG
Spark Blue™ 550 anti-mouse Ly-6G 1A8 FC
Spark NIR™ 685 anti-mouse Ly-6G 1A8 FC
Spark YG™ 593 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
PE/Cyanine5 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
Spark UV™ 387 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 640 anti-mouse Ly-6G 1A8 FC
Spark YG™ 570 anti-mouse Ly-6G 1A8 IHC-F,FC
Spark Red™ 718 anti-mouse Ly-6G (Flexi-Fluor™) 1A8 FC
Spark Blue™ 574 anti-mouse Ly-6G (Flexi-Fluor™) 1A8 FC
Go To Top Version: 2    Revision Date: 01/06/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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