FITC anti-mouse IgD Antibody

Pricing & Availability
Clone
11-26c.2a (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin D
Isotype
Rat IgG2a, κ
11-26Cdot2a_FITC_091907
C57BL/6 mouse splenocytes stained with 11-26c.2a FITC
  • 11-26Cdot2a_FITC_091907
    C57BL/6 mouse splenocytes stained with 11-26c.2a FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability
405703 50 µg $93.00
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405704 500 µg $237.00
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Description

Surface IgD is an important B cell differentiation marker.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.

Application References

(PubMed link indicates BioLegend citation)
  1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
  2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
  3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
  4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
  5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
  7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
  8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
  9. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Rydyznski CE et al. 2018. Cell reports. 24(13):3367-3373 . PubMed
  2. Xu Y, et al. 2022. Nat Commun. 13:980. PubMed
  3. Prior JT, et al. 2023. NPJ Vaccines. 8:80. PubMed
  4. Sweet R, et al. 2011. Proc Natl Acad Sci U S A. 108:7932. PubMed
  5. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  6. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  7. Parthasarathy R, et al. 2021. Front Immunol. 12:758407. PubMed
  8. Duan L, et al. 2021. Immunity. 54:2256. PubMed
  9. Mintz MA, et al. 2019. Immunity. 51:310. PubMed
  10. Liu X, et al. 2022. Nat Cancer. :. PubMed
  11. Zheng S, et al. 2019. PLoS Biol. 17:e3000569. PubMed
  12. Chiu Y, et al. 2014. J Immunol. 193:2207. PubMed
  13. Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed
  14. Suan D et al. 2017. Immunity. 47(6):1142-1153 . PubMed
  15. Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed
  16. Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed
  17. Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed
  18. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  19. Yasuda S, et al. 2018. Genes Cells. 23:868. PubMed
  20. Ryg-Cornejo V, et al. 2016. Cell Rep. 14:68-81. PubMed
  21. Gawish R, et al. 2022. Elife. 11:. PubMed
  22. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  23. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
RRID
AB_315025 (BioLegend Cat. No. 405703)
AB_315025 (BioLegend Cat. No. 405704)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation
Cell Type
B cells
Biology Area
Immunology
Gene ID
380797 View all products for this Gene ID
UniProt
View information about IgD on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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