HRP Goat anti-mouse IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4053 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal IgG
Cat # Size Price Quantity Check Availability
405306 500 µL $141.00
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Description

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Aqueous buffered solution containing a carrier protein and preservatives.
Preparation
The polyclonal antibody was purified from goat antiserum by mouse immunoglobulin affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

To obtain lot-specific expiration date, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

This product has a shelf-life of 12 months or less. Please use our Expiration Lookup Tool to verify the expiration date of your lot of product
Application

ELISA - Quality tested
WB, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. Do not use this product beyond the expiration date. For ELISA or Western blot analysis, the reagent should be titrated between 1:2,000 - 1:5,000 to determine optimal conditions. For IHC on tissue sections, the reagent should be titrated between 1:500 - 1:2000 to determine optimal conditions. It is recommended that the reagent be titrated for optimal performance for each application. Avoid using diluent solutions containg sodium azide. Sodium azide is an inhibitor of horseradish peroxidase.

Application Notes

This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.

Application References

(PubMed link indicates BioLegend citation)
  1. Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed
  2. Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC)
  3. Winters T, et al. 2014. EMBO J. 33:1256-70.
Product Citations
  1. Hajnik RL, et al. 2022. Sci Transl Med. 14:eabq1945. PubMed
  2. Lo NTN, et al. 2022. J Virol. 96:e0066122. PubMed
  3. Knudsen ML, et al. 2022. iScience. 25:105473. PubMed
  4. Thaweerattanasinp T, et al. 2023. PeerJ. 11:e14918. PubMed
  5. Apte S, et al. 2023. Sci Adv. 9:eade1851. PubMed
  6. Weidenbacher PA, et al. 2023. Nat Commun. 14:2149. PubMed
  7. Kaur K, et al. 2023. Front Immunol. 14:1132807. PubMed
  8. Pibuel MA, et al. 2023. Sci Rep. 13:9356. PubMed
  9. Ma Y, et al. 2021. Sci Rep. 11:13471. PubMed
  10. Rutkauskaite J, et al. 2022. iScience. 25:104515. PubMed
  11. Lužnik Marzidovšek Z, et al. 2022. Am J Pathol. 192:270. PubMed
  12. Wright A, et al. 2022. Int J Mol Sci. 23: . PubMed
  13. Hsu J, et al. 2022. J Biol Chem. 299:102848. PubMed
  14. Wang D, et al. 2023. J Exp Clin Cancer Res. 42:26. PubMed
  15. Deich C, et al. 2023. J Biol Eng. 17:4. PubMed
  16. Song E, et al. 2020. bioRxiv. . PubMed
  17. Frank N, et al. 2011. Cancer Res. 71:1474. PubMed
  18. Chen G, et al. 2018. Front Immunol. 9:549. PubMed
  19. Israelow B, et al. 2020. bioRxiv. . PubMed
  20. Uka R, et al. 2020. Oncogene. 39:4132. PubMed
  21. Vrieling M, et al. 2016. Sci Rep. 6:37759. PubMed
  22. Hwang S, et al. 2012. Proc Natl Acad Sci U S A. 110:276. PubMed
  23. Boyt DT, et al. 2020. J R Soc Interface. 17:20190815. PubMed
  24. Lin YR, et al. 2020. Immunity. 53(4):840-851.e6. PubMed
  25. Biering SB et al. 2017. Cell host & microbe. 22(1):74-85 . PubMed
  26. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
  27. Gupta A, et al. 2020. J Biol Chem. 295:2057. PubMed
  28. Copp J, et al. 2014. Proc Natl Acad Sci U S A. 111:13481. PubMed
  29. Chiang CF, et al. 2019. Sci Rep. 9:12448. PubMed
  30. Parks KR, et al. 2019. Cell Rep. 29:3060. PubMed
  31. Baba T, et al. 2021. Cell Death Dis. 12:322. PubMed
  32. Zhang S, et al. 2021. Front Oncol. 585457:11. PubMed
  33. Martinov T, et al. 2019. J Immunol. 203:844. PubMed
  34. Nitiss JL, et al. 2021. Curr Protoc. 1:e250. PubMed
  35. Sharma A, et al. 2021. Traffic. 22:332. PubMed
  36. Yaguchi J, et al. 2021. BMC Biology. 19(1):64. PubMed
  37. Ventura E, et al. 2018. J Cell Sci. 131. PubMed
  38. Collin R, et al. 2014. J Immunol. 193:3503. PubMed
  39. Song E, et al. 2021. J Exp Med. 218:00:00. PubMed
  40. Nishigami Y, et al. 2016. Sci Rep. 6:18964. PubMed
  41. Omer S et al. 2018. eLife. 7 pii: e36745. PubMed
  42. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  43. Hoffmann D, et al. 2020. Cancer Immunol Res. 8:19. PubMed
  44. Liu H, et al. 2020. Cell Death Dis. 0.767361111. PubMed
  45. Pibuel MA, et al. 2021. Cell Death Discov. 7:280. PubMed
  46. Furlong K, et al. 2020. J Virol. 94:00:00. PubMed
  47. Yan L, et al. 2014. Clin Vaccine Immunol. 21:1206. PubMed
  48. Mamedov T, et al. 2021. Front Plant Sci. 12:742875. PubMed
  49. Fang Z, et al. 2022. Nat Commun. 13:3250. PubMed
  50. Song E, et al. 2021. Cell Rep Med. 2:100288. PubMed
  51. Lee K, et al. 2020. Biology (Basel). 9:00. PubMed
  52. Powell AE, et al. 2020. bioRxiv. . PubMed
  53. Powell AE, et al. 2022. Front Immunol. 13:942897. PubMed
  54. Wang K, et al. 2021. EMBO Rep. 22:e52205. PubMed
  55. Gopinath A, et al. 2022. NPJ Parkinsons Dis. 8:72. PubMed
  56. Ohtsuka J, et al. 2021. iScience. Online ahead of print. PubMed
  57. Harbour JC, et al. 2021. J Immunol. 206:2596. PubMed
  58. Gopinath A, et al. 2021. NPJ Parkinsons Dis. 7:62. PubMed
  59. Seydoux E, et al. 2021. Cell Reports. 35(5):109084. PubMed
  60. Caglar Beker M, et al. 2020. Turk J Med Sci. Online ahead of print. PubMed
  61. Hsing C, et al. 2008. Cytokine. 44:221. PubMed
  62. Sertorio M, et al. 2016. J Immunol. 196: 2986-2994. PubMed
  63. Hsu HW, et al. 2020. Development. 147:00:00. PubMed
  64. Wang C, et al. 2019. PLoS Pathog. 15:e1007938. PubMed
  65. Khan AA, et al. 2022. Bioconjug Chem. . PubMed
  66. Ram AK, et al. 2020. World J Gastroenterol. 26:7131. PubMed
  67. Chung KP, et al. 2019. Nat Commun. 10:3390. PubMed
  68. Jin SW, et al. 2019. Cell Rep. 29:1449. PubMed
  69. Zhang Q, et al. 2021. Front Cell Infect Microbiol. 11:766922. PubMed
  70. Lee SH, et al. 2022. Nat Commun. 13:5461. PubMed
  71. Shkoporov A, et al. 2015. FEMS Microbiol Lett. 362:83. PubMed
  72. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  73. Kiianitsa K, et al. 2020. DNA Repair (Amst). 96:102977. PubMed
  74. Viereckl MJ, et al. 2022. Biomolecules. 12:. PubMed
  75. Klaessens S, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  76. Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed
  77. Prakash P, et al. 2019. Methods Protoc. 2:48. PubMed
  78. Wu YH, et al. 2020. Mol Med Rep. 21:851. PubMed
  79. Powell AE, et al. 2021. ACS Cent Sci. 7:183. PubMed
  80. Tao L, et al. 2021. Light Sci Appl. 10:179. PubMed
  81. Horton N, et al. 2013. PLoS One. 8:e59552. PubMed
  82. Nanna AR, et al. 2020. Nucleic Acids Res. 48:5281. PubMed
  83. Ortega-Prieto AM, et al. 2018. Nat Commun. 9:682. PubMed
  84. Liang YH, et al. 2021. J Biomed Sci. 28:75. PubMed
  85. Lun Y, et al. 2021. J Immunol. 206:2819. PubMed
  86. Rodems TS, et al. 2022. Commun Biol. 5:897. PubMed
  87. Hoffmann D, et al. 2020. Int J Tryptophan Res. 12:1178646919891736. PubMed
  88. Zhang B, et al. 2020. J Exp Med. 217:e20182365. PubMed
  89. Miyamoto M, et al. 2018. Oncol Lett. 15:4005. PubMed
  90. Han G, et al. 2022. Ann Transl Med. 10:1134. PubMed
  91. Harbuzariu A, et al. 2022. iScience. 25:104407. PubMed
  92. Huang WC, et al. 2022. Sci Rep. 12:3612. PubMed
  93. Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed
RRID
AB_315009 (BioLegend Cat. No. 405306)

Other Formats

View All IgG Reagents Request Custom Conjugation
Description Clone Applications
APC Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Biotin Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
FITC Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
HRP Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ELISA,IHC,WB
PE Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Purified Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ELISA,FC,WB,IHC
Cyanine3 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ICC
PE/Cyanine7 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
PerCP/Cyanine5.5 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
DyLight™ 488 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,ICC
DyLight™ 649 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Alexa Fluor® 594 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ICC,IHC-F,IHC-P
APC/Cyanine7 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Brilliant Violet 421™ Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,ICC
Alexa Fluor® 488 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,IHC-F
Alexa Fluor® 647 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,IHC-P
Alexa Fluor® 555 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ICC
Brilliant Violet 605™ Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Brilliant Violet 510™ Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
PE/Dazzle™ 594 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
PerCP Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
APC/Fire™ 750 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Spark YG™ 570 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,IHC-P
Spark Red™ 718 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 IHC-P,IHC-F,ICC
Go To Top Version: 6    Revision Date: 12/12/2022

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