Pacific Blue™ anti-mouse IgD Antibody

Pricing & Availability
Clone
11-26c.2a (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin D
Isotype
Rat IgG2a, κ
A_11-26cdot2a_PB_102809
C57BL/6 splenocytes stained with 11-26c.2a Pacific Blue™ and CD19 antibody (6D5) PE
  • A_11-26cdot2a_PB_102809
    C57BL/6 splenocytes stained with 11-26c.2a Pacific Blue™ and CD19 antibody (6D5) PE
  • B_11-26cdot2a_PB_102809
    C57BL/6 splenocytes stained with rat IgG2a Pacific Blue™ isotype and CD19 antibody (6D5) PE
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability
405711 25 µg $106.00
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405712 100 µg $223.00
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Description

Surface IgD is an important B cell differentiation marker.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.

Application References

(PubMed link indicates BioLegend citation)
  1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
  2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
  3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
  4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
  5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
  7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
  8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
  9. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed
  2. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  3. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  4. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  5. Shikatani EA et al. 2019. Cell Rep. 27(8):2304-2312 . PubMed
  6. Dave K et al. 2017. eLife. 6 pii: e23382. PubMed
  7. Kim Y, et al. 2015. PLoS One. 10:120294. PubMed
  8. Mathew NR, et al. 2021. Cell Reports. 35(12):109286. PubMed
  9. Holodick N, et al. 2014. J Immunol. 192:2432. PubMed
  10. Muppidi J, et al. 2015. J Exp Med. 212: 2213 - 2222. PubMed
  11. Duan L, et al. 2021. Immunity. 54:2256. PubMed
  12. McGuire A, et al. 2016. Nat Commun. 7:10618. PubMed
  13. Chen M, et al. 2014. PLoS One. 9:107505. PubMed
  14. Ray A, et al. 2015. PLoS One. 10: 0137314. PubMed
  15. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  16. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
  17. Holodick N, et al. 2016. J Immunol. 196: 4348 - 4357. PubMed
  18. Olson WJ, et al. 2019. Cell Rep. 28:2878. PubMed
  19. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  20. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  21. Clancy–Thompson E, et al. 2019. EMBO J. 38:e101260. PubMed
  22. Vijay R, et al. 2021. J Exp Med. 218:. PubMed
  23. Wright JA, et al. 2021. Front Immunol. 12:705307. PubMed
  24. Vijay R, et al. 2020. Nat Immunol. 21:790. PubMed
  25. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  26. Ang QY, et al. 2020. Cell. 181(6):1263-1275.e16. PubMed
RRID
AB_1937244 (BioLegend Cat. No. 405711)
AB_1937244 (BioLegend Cat. No. 405712)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation
Cell Type
B cells
Biology Area
Immunology
Gene ID
380797 View all products for this Gene ID
UniProt
View information about IgD on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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