PE/Cyanine7 anti-mouse IgM Antibody

Pricing & Availability
Clone
RMM-1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin M
Isotype
Rat IgG2a, κ
RMM-1_PECyanine7_IgM_Antibody_1_041024
C57BL/6 splenocytes stained with CD19 (6D5) APC and IgM (clone RMM-1) PE/Cyanine7 (left) or rat IgG2a isotype control (right).
  • RMM-1_PECyanine7_IgM_Antibody_1_041024
    C57BL/6 splenocytes stained with CD19 (6D5) APC and IgM (clone RMM-1) PE/Cyanine7 (left) or rat IgG2a isotype control (right).
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability
406513 25 µg $89.00
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406514 100 µg $229.00
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Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Application References

(PubMed link indicates BioLegend citation)
  1. Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
Product Citations
  1. Hong H, et al. 2022. STAR Protoc. 3:101526. PubMed
  2. Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed
  3. Thomsen I, et al. 2021. J Immunol. Online ahead of print. PubMed
  4. Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed
  5. MacLean AJ, et al. 2022. Immunity. 55:718. PubMed
  6. Erickson JJ, et al. 2022. Nature. 606:769. PubMed
  7. Blanco LP, et al. 2022. Arthritis Rheumatol. 74:1971. PubMed
  8. Urata S, et al. 2023. Microbiol Immunol. 67:105. PubMed
  9. Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed
  10. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  11. Hung PJ, et al. 2018. Mol Cell. 71:332. PubMed
  12. Brestoff JR, et al. 2021. Cell Metab. 33:270. PubMed
  13. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  14. Cunha P, et al. 2022. Front Vet Sci. 9:891893. PubMed
  15. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  16. Fredriksson-Lidman K, et al. 2017. PLoS One. 10.1371/journal.pone.0185509. PubMed
  17. Sauma D, et al. 2017. Immunol Res. 10.1007/s12026-017-8936-9. PubMed
  18. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  19. Li H, et al. 2020. Nature. 584:274. PubMed
  20. Jonas B, et al. 2016. PLoS One. 11: 0159189. PubMed
  21. Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed
  22. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  23. Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed
  24. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  25. Staffas A et al. 2018. Cell host & microbe. 23(4):447-457 . PubMed
  26. Habets T, et al. 2016. PLoS One. 11: 0159515. PubMed
  27. Schardt JS, et al. 2021. Sci Rep. 11:20738. PubMed
  28. Nagatake T, et al. 2019. Mucosal Immunol. 12:p1082. PubMed
RRID
AB_10642031 (BioLegend Cat. No. 406513)
AB_10642031 (BioLegend Cat. No. 406514)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type
B cells
Biology Area
Immunology
Gene ID
16019 View all products for this Gene ID
UniProt
View information about IgM on UniProt.org
Go To Top Version: 2    Revision Date: 01/29/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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