PerCP/Cyanine5.5 anti-mouse IgG1 Antibody

Pricing & Availability
Clone
RMG1-1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G1
Isotype
Rat IgG
RMG1-1_PerCPCyanine55_IgG1_Antibody_101818
Human peripheral blood lymphocytes were stained with purified anti-human CD3 (clone UCHT1, filled histogram), or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG1 (clone RMG1-1) PerCP/Cyanine5.5.
  • RMG1-1_PerCPCyanine55_IgG1_Antibody_101818
    Human peripheral blood lymphocytes were stained with purified anti-human CD3 (clone UCHT1, filled histogram), or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG1 (clone RMG1-1) PerCP/Cyanine5.5.
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability
406611 25 µg $89.00
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406612 100 µg $194.00
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Description

The RMG1-1 monoclonal antibody reacts with immunoglobulin G1 (IgG1) in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG1-1 monoclonal antibody may be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Rucci F, et al. 2010. P. Natl. Acad. Sci. USA 107:3024. (FC) PubMed
  2. Zhang JG, et al. 2011. Int. Immunopharmacol. 11:1685. PubMed
Product Citations
  1. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  2. Jang A, et al. 2021. Front Immunol. 12:701066. PubMed
RRID
AB_2562000 (BioLegend Cat. No. 406611)
AB_2562000 (BioLegend Cat. No. 406612)
Go To Top Version: 2    Revision Date: 10/18/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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