- Clone
- Poly4054 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Goat Polyclonal IgG
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 405424 | 100 µg | $253.00 | |||
Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.
Product Details
- Verified Reactivity
- Rat
- Antibody Type
- Polyclonal
- Host Species
- Goat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.
-
Application References
(PubMed link indicates BioLegend citation) - RRID
-
AB_2563515 (BioLegend Cat. No. 405424)
Other Formats
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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APC Goat anti-rat IgG (minimal x-reactivity)
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FITC Goat anti-rat IgG (minimal x-reactivity)
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HRP Goat anti-rat IgG (minimal x-reactivity)
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PE Goat anti-rat IgG (minimal x-reactivity)
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Purified Goat anti-rat IgG (minimal x-reactivity)
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PE/Cyanine7 Goat anti-rat IgG (minimal x-reactivity)
C57BL/c mouse splenocytes stained with purified rat anti-mou... -
Cyanine3 Goat anti-rat IgG (minimal x-reactivity)
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Brilliant Violet 421™ Goat anti-rat IgG (minimal x-reactivity)
293E cells were stained with purified anti-Histone H3-Phosph... -
Alexa Fluor® 647 Goat anti-rat IgG (minimal x-reactivity)
Human frozen tonsil section was fixed with 4% paraformaldehy... -
Alexa Fluor® 488 Goat anti-rat IgG (minimal x-reactivity)
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
Alexa Fluor® 555 Goat anti-rat IgG (minimal x-reactivity)
NIH/3T3 mouse fibroblasts were fixed with 1% paraformaldehyd... 
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
Alexa Fluor® 594 Goat anti-rat IgG (minimal x-reactivity)
NIH/3T3 mouse fibroblast cell line was fixed with 1% parafor... 
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
PerCP/Cyanine5.5 Goat anti-rat IgG (minimal x-reactivity)
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APC/Fire™ 750 Goat anti-rat IgG (minimal x-reactivity)
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Brilliant Violet 605™ Goat anti-rat IgG (minimal x-reactivity)
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PE/Dazzle™ 594 Goat anti-rat IgG (minimal x-reactivity)
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Biotin Goat anti-rat IgG (minimal x-reactivity)