Purified anti-mouse IgM (Maxpar® Ready) Antibody

Pricing & Availability
Clone
RMM-1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin M
Isotype
Rat IgG2a, κ
RMM-1_Purified_IgM_Cytof_061014.jpg
C57BL/6 mouse splenocytes stained with 176Yb-anti-CD45R/B220 (RA36B2) and 151Eu-anti-IgM (RMM-1). Data provided by DVS Sciences.
  • RMM-1_Purified_IgM_Cytof_061014.jpg
    C57BL/6 mouse splenocytes stained with 176Yb-anti-CD45R/B220 (RA36B2) and 151Eu-anti-IgM (RMM-1). Data provided by DVS Sciences.
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Cat # Size Price Quantity Check Availability
406527 100 µg $106.00
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Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The IgM antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References

(PubMed link indicates BioLegend citation)
  1. Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
Product Citations
  1. Dasgupta D, et al. 2020. Gastroenterology. 159:1487. PubMed
  2. Balog JA, et al. 2021. Genomics Proteomics Bioinformatics. 19:243. PubMed
RRID
AB_2563777 (BioLegend Cat. No. 406527)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type
B cells
Biology Area
Immunology
Gene ID
16019 View all products for this Gene ID
UniProt
View information about IgM on UniProt.org
Go To Top Version: 2    Revision Date: 07/22/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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