Purified Goat anti-rat IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4054 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal IgG
Cat # Size Price Quantity Check Availability
405401 500 µg $220.00
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Description

Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.

Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA - Quality tested
FC, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture, the suggested use of this reagent is ≤ 2.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.

Application References

(PubMed link indicates BioLegend citation)
  1. Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed
  2. Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed
  3. Baba T, et al. 2020. eLife. 9:e54257. (IHC-P)
Product Citations
  1. Wang L, et al. 2022. J Gen Physiol. 154:. PubMed
  2. Liu PS, et al. 2023. Nat Immunol. 24:452. PubMed
  3. Karulf M, et al. 2010. J Immunol. 185:4856. PubMed
RRID
AB_315012 (BioLegend Cat. No. 405401)
Go To Top Version: 4    Revision Date: 02/24/2023

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