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Alexa Fluor® 700 anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1dot4_AF700_072210
C57BL/6 bone marrow cells stained with HK1.4 Alexa Fluor® 700
  • HK1dot4_AF700_072210
    C57BL/6 bone marrow cells stained with HK1.4 Alexa Fluor® 700
Compare all formats See Alexa Fluor® 700 spectral data
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128023 25 µg $124
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128024 100 µg $265
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  2. Lee JH, et al. 2022. EBioMedicine. 77:103903. PubMed
  3. Miró L, et al. 2022. Cancers (Basel). 14: . PubMed
  4. Domingos-Pereira S, et al. 2022. Int J Mol Sci. 24: . PubMed
  5. Drummer C, et al. 2023. Front Immunol. 14:1113883. PubMed
  6. Khan NZ, et al. 2021. Brain Behav Immun. 92:165. PubMed
  7. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  8. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  9. Le DT, et al. 2023. iScience. 26:106059. PubMed
  10. Ivan DC, et al. 2023. Acta Neuropathol Commun. 11:35. PubMed
  11. Xu H, et al. 2023. Elife. 12:. PubMed
  12. Vanneste D, et al. 2023. Nat Immunol. 24:827. PubMed
  13. Wang X, et al. 2023. Stem Cell Rev Rep. . PubMed
  14. Carone M, et al. 2023. Nat Commun. 14:3489. PubMed
  15. Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
  16. Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed
  17. Kolawole A, et al. 2015. J Virol. 90: 1499-1506. PubMed
  18. Denk D, et al. 2022. Immunity. 55:2059. PubMed
  19. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
  20. Minutti CM, et al. 2019. Immunity. 50:645. PubMed
  21. Lindborg JA et al. 2017. The Journal of Neuroscience. 37(43):10258-10277 . PubMed
  22. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  23. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  24. Zhang X, et al. 2020. Elife. 9:00. PubMed
  25. Parrish KE, et al. 2021. Biopharm Drug Dispos. 42:137. PubMed
  26. Li Y, et al. 2021. Brain Behav Immun. 91:267. PubMed
  27. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  28. Deng M, et al. 2020. Nat Commun. 11:2193. PubMed
  29. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  30. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
  31. Ritzel RM, et al. 2021. Glia. 69:746. PubMed
  32. Otvos B, et al. 2021. Clin Cancer Res. 27:2038. PubMed
  33. Hu Y, et al. 2021. Cell Death Dis. 12:743. PubMed
  34. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  35. Chhatbar C et al. 2018. Cell reports. 25(1):118-129 . PubMed
  36. Nacer A, et al. 2014. PLoS Pathog. 10:1004528. PubMed
  37. Velázquez F, et al. 2016. J Immunol. 196: 1305 - 1316. PubMed
  38. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  39. Issah Y, et al. 2021. Elife. 10:. PubMed
  40. Ortega-Molina A, et al. 2021. Cell Reports. 36(2):109372. PubMed
  41. Tähtinen S, et al. 2022. Nat Immunol. 23:532. PubMed
  42. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
  43. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  44. Dyer DP et al. 2019. Immunity. 50(2):378-389 . PubMed
  45. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  46. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
  47. Buschor S, et al. 2017. PLoS Pathogens. 13(6):e1006476. PubMed
RRID
AB_10643270 (BioLegend Cat. No. 128023)
AB_10643270 (BioLegend Cat. No. 128024)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org
Go To Top Version: 3    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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