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APC/Cyanine7 anti-human CD3 Antibody

Pricing & Availability
Clone
HIT3a (See other available formats)
Regulatory Status
RUO
Workshop
V CD03.05
Other Names
T3, CD3ε
Isotype
Mouse IgG2a, κ
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Product Citations
publications
HIT3a_APCCy7_122007
Human peripheral blood lymphocytes stained with HIT3a APC/Cyanine7
  • HIT3a_APCCy7_122007
    Human peripheral blood lymphocytes stained with HIT3a APC/Cyanine7
Compare all formats See APC/Cyanine7 spectral data
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300317 25 tests $153
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300318 100 tests $311
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Sedelies KA, et al. 2004. J. Biol. Chem. 279:26581. (Activ)
  5. Rivollier A, et al. 2004. Blood 104:4029. (Activ)
  6. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Activ)
  7. Smeltz RB. 2007. J. Immunol. 178:4786. (Activ)
Product Citations
  1. Howson LJ, et al. 2018. Nat Commun. 9:253. PubMed
  2. Tocheva AS, et al. 2020. Curr Protoc Immunol. 130:e103. PubMed
  3. Aoki T, et al. 2020. Cancer Sci. 111:2223. PubMed
  4. Sun A, et al. 2021. J Dent Sci. 16:751. PubMed
  5. Schneider MM, et al. 2022. Life Sci Alliance. 5: . PubMed
  6. Stunnenberg M, et al. 2022. J Leukoc Biol. 112:289. PubMed
  7. Shemesh A, et al. 2022. J Exp Med. 219: . PubMed
  8. Kerns JS, et al. 2023. Bio Protoc. 13: . PubMed
  9. Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed
  10. Tate T, et al. 2023. Cancers (Basel). 15:. PubMed
  11. Tang L, et al. 2023. Front Immunol. 14:1145441. PubMed
  12. Nakano Y, et al. 2017. PLoS Pathog. 13:e1006348. PubMed
  13. Hagan T, et al. 2020. Cell. 178(6):1313-1328.e13.. PubMed
  14. Hearnden R, et al. 2021. STAR Protocols. 2(2):100422. PubMed
  15. Causi E, et al. 2015. PLoS One. 10: 0136717. PubMed
  16. Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed
  17. Nixon CC, et al. 2020. Cell Reports Medicine. 578(7793):160-165. PubMed
  18. Kramer KJ, et al. 2022. Nat Commun. 13:3466. PubMed
  19. Yamaguchi A, et al. 2021. J Thorac Dis. 13:5430. PubMed
  20. Hinderer S, et al. 2012. Biomaterials. 33:5259. PubMed
  21. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  22. Liu R, et al. 2021. Commun Biol. 4:652. PubMed
  23. Kreutmair S, et al. 2021. Immunity. . PubMed
  24. Khanam A, et al. 2021. Front Immunol. 11:599648. PubMed
  25. Gerstner S, et al. 2016. J Leukoc Biol. 100(6):1297-1310. PubMed
  26. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  27. Neff CP et al. 2018. EBioMedicine. 30:192-202 . PubMed
  28. Phad GE, et al. 2022. Nat Immunol. 23:1. PubMed
  29. Basar R, et al. 2021. Cell Reports. 36(3):109432. PubMed
  30. Shen J, et al. 2021. Sci Adv. 7:eabi9787. PubMed
  31. Lerrer S, et al. 2021. iScience. 24:103020. PubMed
  32. Stunnenberg M, et al. 2021. Eur J Immunol. 51:2464. PubMed
  33. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  34. Evans WS, et al. 2020. Exp Physiol. 105:1408. PubMed
  35. Ohue Y, et al. 2014. Clin Cancer Res. 20:5052. PubMed
  36. Bradley S, et al. 2015. PLoS One. 10: 0141171. PubMed
  37. Wang F, et al. 2021. Cell. 184(2):422-440.e17. PubMed
  38. Pache L, et al. 2020. Cell Rep Med. 1:100037. PubMed
  39. Vikkurthi R, et al. 2022. Nat Microbiol. 7:974. PubMed
  40. Su X, et al. 2022. J Transl Med. 20:378. PubMed
  41. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  42. Kim D, et al. 2020. Nat Med. 26:236. PubMed
  43. Liu Y, et al. 2020. Methods Mol Biol. 2111:285. PubMed
  44. Shemesh A, et al. 2022. J Exp Med. 219:. PubMed
  45. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  46. Duque JSR, et al. 2022. Nat Commun. 13:3700. PubMed
  47. Harb H, et al. 2020. Nat Immunol. 1359:21. PubMed
  48. Liu Y, et al. 2015. J Immunol. 194:5851. PubMed
  49. Sato K, et al. 2014. PLoS Pathog. 10:1004453. PubMed
  50. Younis R, et al. 2016. J Immunol. 196: 1419 - 1429. PubMed
  51. Singh KS, et al. 2021. Nature. 589:597. PubMed
  52. Clayton KL, et al. 2021. Cell Host Microbe. 29(3):435-447.e9. PubMed
  53. Koh WH, et al. 2020. STAR Protoc. 1:100203. PubMed
  54. Chakraborty A, et al. 2022. Methods Mol Biol. 2442:565. PubMed
  55. Olafsdottir TA, et al. 2022. Commun Biol. 5:914. PubMed
  56. Gladkikh A, et al. 2017. Cancer Med. . 10.1002/cam4.1257. PubMed
  57. Li M, et al. 2020. Virol Sin. 35:588. PubMed
  58. Friedensohn S, et al. 2018. Front Immunol. 9:1401. PubMed
  59. Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
  60. Feng Y, et al. 2022. EClinicalMedicine. 43:101226. PubMed
RRID
AB_314053 (BioLegend Cat. No. 300317)
AB_314053 (BioLegend Cat. No. 300318)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK-T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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