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- Clone
- A019D5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-7 receptor α chain, IL-7Rα
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human PBMCs stained with 154Sm-anti-CD3 (UCHT1) and 143Nd-anti-CD127 (A019D5). CD45+ CD33- cells are displayed in the analysis. Data provided by DVS Sciences.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 351337 | 100 µg | $147 | ||||
CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human CD127
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF®, PG - Verified - Recommended Usage
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This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
-
Additional reported (for the relevant formats) application: proteogenomics1.
- Additional Product Notes
-
Maxpar® is a registered trademark of Standard BioTools Inc.
-
Application References
(PubMed link indicates BioLegend citation) -
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
-
- RRID
-
AB_2563715 (BioLegend Cat. No. 351337)
Antigen Details
- Structure
- Type I transmembrane glycoprotein, associates with CD132, 60-90 kD
- Distribution
-
Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
- Function
- T cell and immature B cell proliferation and development
- Ligand/Receptor
- IL-7
- Cell Type
- B cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503.
3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610.
4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.
6. Liu W, et al. 2006. J. Exp. Med. 203:1701. - Gene ID
- 3575 View all products for this Gene ID
- UniProt
- View information about CD127 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
-
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
-
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
-
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All CD127 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with purifie... -
PE anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 APC... 
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Pacific Blue™ anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 PE ... 
-
Brilliant Violet 421™ anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 FIT... 
-
FITC anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 APC... 
-
Alexa Fluor® 488 anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 APC... 
-
APC anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 FIT... 
-
Alexa Fluor® 647 anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 FIT... -
PE/Cyanine7 anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 FIT... -
PerCP/Cyanine5.5 anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Brilliant Violet 570™ anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 FIT... 
-
PE/Cyanine5 anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Brilliant Violet 650™ anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Brilliant Violet 711™ anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD127 (... -
Brilliant Violet 785™ anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD127 (... -
Brilliant Violet 510™ anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD127 (... -
Brilliant Violet 605™ anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 FIT... 
-
PE/Dazzle™ 594 anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 FIT... 
-
Purified anti-human CD127 (IL-7Rα) (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD3 (UCHT1) and 143Nd-an... -
Alexa Fluor® 700 anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 PE ... 
-
Biotin anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 APC... 
-
APC/Cyanine7 anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 FIT... -
APC/Fire™ 750 anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 FIT... 
-
TotalSeq™-A0390 anti-human CD127 (IL-7Rα)
-
TotalSeq™-B0390 anti-human CD127 (IL-7Rα)
-
TotalSeq™-C0390 anti-human CD127 (IL-7Rα)
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KIRAVIA Blue 520™ anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 APC... -
Spark NIR™ 685 anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes stained with CD3 FITC and... -
PE/Fire™ 640 anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 700 anti-human CD127 (IL-7Rα) Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark YG™ 581 anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 750™ anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-D0390 anti-human CD127 (IL-7Rα)
-
APC/Fire™ 810 anti-human CD127 (IL-7Rα) Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
APC/Fire™ 750 anti-human CD127
Typical results from human peripheral blood lymphocytes stai... -
PE anti-human CD127
Typical results from human peripheral blood lymphocytes stai... -
PerCP/Cyanine5.5 anti-human CD127
Typical results from human peripheral blood lymphocytes stai... -
PE/Cyanine7 anti-human CD127
Typical results from human peripheral blood lymphocytes stai... -
Spark Red™ 718 anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PE/Cyanine7 anti-human CD127 (IL-7Rα)
Typical results from human peripheral blood lymphocytes stai... -
PE/Fire™ 744 anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Fire™ 780 anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Fire™ 806 anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark PLUS B550™ anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with anti-hu...
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