Loading...

Purified anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
Compare all formats
Cat # Size Price Quantity Check Availability Save
123101 50 µg 47 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
123102 500 µg 258 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

F4/80, also known as EMR1 or Ly71, is a 160 kD glycoprotein of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80 has been widely used as a murine macrophage marker. It is expressed on a majority of tissue macrophages, including macrophages in the lung, gut, peritoneal cavity, thymus, and red pulp of the spleen, Kupffer cells, Langerhans cells, microglia, and certain dendritic cells. It is not expressed on macrophages located in the T cell areas of the spleen, lymph node, or Peyer's patch. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for the induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Hernández-Santana YE, et al. 2020. Life Sci Alliance. 3:00. PubMed
  2. Rehrauer H, et al. 2018. Oncogene. 7:1487250. PubMed
  3. Gomes AC, et al. 2019. J Immunol. 203:2485. PubMed
  4. Dallari S, et al. 2017. Nat Commun. 8:14830. PubMed
  5. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  6. Elshal M, et al. 2022. Arch Pharm Res. . PubMed
  7. Rosina M, et al. 2022. Cell Metab. 34:533. PubMed
  8. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  9. Volmari A, et al. 2021. Hepatol Commun. 5:2104. PubMed
  10. Li F, et al. 2020. EMBO J. 39:e103205. PubMed
  11. Liu CJ, et al. 2020. Neuro Oncol. 22:1276. PubMed
  12. Calcagno DM, et al. 2020. Sci Immunol. 5: . PubMed
  13. Marneros AG, et al. 2023. FEBS J. 290:28. PubMed
  14. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  15. Valet C, et al. 2022. J Clin Invest. 132: . PubMed
  16. Fu A, et al. 2022. Cell. 185:1356. PubMed
  17. Marquardt RM, et al. 2022. Int J Mol Sci. 23: . PubMed
  18. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  19. Wang JX, et al. 2023. JCI Insight. 8:. PubMed
  20. Bautista CA, et al. 2023. Front Physiol. 14:1122348. PubMed
  21. Zhao C, et al. 2023. NPJ Regen Med. 8:29. PubMed
  22. Reglero-Real N, et al. 2021. Immunity. :. PubMed
  23. Kaneko T, et al. 2012. J Immunol. 188:5397. PubMed
  24. Vitiello GA, et al. 2018. Clin Cancer Res. 24:972. PubMed
  25. Burrello C, et al. 2018. Front Med (Lausanne). 5:21. PubMed
  26. Shirakawa K, et al. 2017. PLoS One. 12:e0186303. PubMed
  27. Gray ME, et al. 2018. J Biomed Mater Res B Appl Biomater. 107(5):1620-1633. PubMed
  28. Wang B et al. 2018. Cell stem cell. 22(2):206-220 . PubMed
  29. Zou B, et al. 2017. PLoS Pathogens. 13(6):e1006436. PubMed
  30. Cheng AA, et al. 2021. Am J Physiol Endocrinol Metab. 320:E438. PubMed
  31. Nobs SP, et al. 2021. J Allergy Clin Immunol. . PubMed
  32. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  33. Maestro S, et al. 2021. Viruses. 13: . PubMed
  34. Chen YS, et al. 2019. Cells. 8:3. PubMed
  35. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  36. Karnezis T et al. 2019. Cancer Res. 79(7):1558-1572 . PubMed
  37. Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
  38. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  39. Duvvuri M, et al. 2019. Biomater Sci. 7:1863. PubMed
  40. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  41. Accarias S, et al. 2020. J Cell Sci. 133:. PubMed
  42. Prado C, et al. 2021. J Neuroinflammation. 18:292. PubMed
  43. Lu L, et al. 2022. Sci Rep. 12:931. PubMed
  44. Yang J, et al. 2022. Biosci Rep. :. PubMed
  45. Elshal M, et al. 2022. Inflammopharmacology. :. PubMed
  46. Altshuler A, et al. 2021. Cell Stem Cell. 28(7):1248-1261.e8. PubMed
  47. Yuan Q, et al. 2021. Cell Reports. 34(5):108724. PubMed
  48. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  49. Chen J, et al. 2018. Hypertension. 71:877. PubMed
  50. Weis S et al. 2017. Cell. 169(7):1263-1275 . PubMed
  51. De Vlaeminck Y, et al. 2020. Cancers (Basel). 12:. PubMed
  52. Xu Q, et al. 2021. Front Physiol. 12:732084. PubMed
  53. Kennedy CL, et al. 2021. Toxics. 9:. PubMed
  54. Goswami S, et al. 2021. J Cell Physiol. 236:8148. PubMed
  55. Cai Z, et al. 2021. Front Immunol. 12:646384. PubMed
  56. Nagatake T, et al. 2022. Mucosal Immunol. 15:289. PubMed
  57. Wada N, et al. 2020. Mol Metab. 37:100988. PubMed
  58. Malsy J, et al. 2020. Elife. 9:00. PubMed
  59. Cozzitorto C, et al. 2020. Developmental Cell. 55(2):150-162.e6. PubMed
  60. Wang Y, et al. 2021. Cancer Cell. 39:1375. PubMed
  61. Ma F, et al. 2020. Sci Signal. :13. PubMed
  62. Motoyama S, et al. 2020. Cells. :9. PubMed
  63. Cheng Y, et al. 2018. J Exp Med. 215:2919. PubMed
  64. Arimura K, et al. 2017. Mucosal Immunol. 1.08125. PubMed
  65. Duarte N, et al. 2018. Hepatol Commun. 0.833333333. PubMed
  66. Liu T, et al. 2020. Sci Adv. 6:eaay1497. PubMed
  67. Cvetanova B, et al. 2021. Int J Mol Sci. 22: . PubMed
  68. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  69. Huang YL, et al. 2021. Antioxidants (Basel). 10:. PubMed
  70. Peng J, et al. 2020. Toxicol Res (Camb). 9:609. PubMed
  71. Huang YL, et al. 2021. Aging Cell. 20:e13523. PubMed
  72. Stefanescu C, et al. 2021. Front Oncol. 11:765151. PubMed
  73. Kuziel G, et al. 2020. Cancers (Basel). 12:00. PubMed
  74. Hayakawa K, et al. 2016. J Cereb Blood Flow Metab. 36: 781-793. PubMed
  75. Do DC, et al. 2019. JCI Insight. 4:e126832. PubMed
  76. Kitai Y, et al. 2017. J Immunol. 198:1649. PubMed
  77. Nakamizo S,et al. 2017. Sci Rep. . 10.1038/s41598-017-14292-1. PubMed
  78. Yang M, et al. 2020. Oncoimmunology. 9:1708064. PubMed
  79. Miyake M, et al. 2021. iScience. 24:103448. PubMed
  80. Billerhart M, et al. 2021. Mol Ther Oncolytics. 23:192. PubMed
  81. Sun H, et al. 2018. J Cell Biol. 217:1453. PubMed
  82. Bálint L, et al. 2020. Front Immunol. 2.529861111. PubMed
  83. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  84. O'Dea KP, et al. 2020. J Extracell Vesicles. 9:1706708. PubMed
  85. Matsuhira T, et al. 2021. Biochem Biophys Rep. 28:101118. PubMed
  86. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
  87. Li Y, et al. 2021. Cardiovasc Res. 890:117. PubMed
  88. Cassidy LD, et al. 2018. Autophagy. 1.455555556. PubMed
  89. Seelige R, et al. 2018. Sci Rep. 8:13670. PubMed
  90. Tsujinaka H, et al. 2020. Nat Commun. 0.940277778. PubMed
  91. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  92. Rätsep MT, et al. 2018. Am J Physiol Lung Cell Mol Physiol. 315:L977. PubMed
  93. Matsuo R, et al. 2022. JID Innov. 2:100141. PubMed
  94. Sun Y, et al. 2022. Nat Commun. 13:3916. PubMed
  95. Shaker ME, et al. 2022. Saudi Pharm J. 30:291. PubMed
  96. Oba T, et al. 2021. J Immunother Cancer. 9:. PubMed
  97. Yang K, et al. 2017. Nature. 548:602. PubMed
  98. Mainini F, et al. 2018. Nucleic Acid Ther. 28:225. PubMed
  99. Kobayashi M, et al. 2008. J Leukoc Biol. 83:1354. PubMed
  100. Andersson &, et al. 2011. Clin Cancer Res. 3.25. PubMed
  101. Sato Y, et al. 2021. BMC Cancer. 21:1222. PubMed
  102. Wang Y, et al. 2021. Sci Transl Med. 13:. PubMed
  103. Shokirova H, et al. 2021. Sci Rep. 11:8647. PubMed
  104. Wei S, et al. 2019. Virulence. 0.588888889. PubMed
  105. Borges TJ, et al. 2018. Nat Commun. 9:3482. PubMed
  106. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  107. Mueller–Ortiz SL, et al. 2019. J Immunol. 203:2701. PubMed
  108. Kaufmann B, et al. 2022. EMBO Rep. 23:e54446. PubMed
  109. Hu G, et al. 2021. Nat Commun. 12:773. PubMed
  110. Tsai F, et al. 2017. J Exp Med. 214:3753. PubMed
  111. Davies CL, et al. 2019. Front Immunol. 10:1048. PubMed
  112. Shin J, et al. 2018. Diabetes. 67:1068. PubMed
  113. Janardhan KS, et al. 2018. Toxicol Pathol. 46:488. PubMed
  114. Johnson SA, et al. 2021. Eur J Immunol. 51:3228. PubMed
  115. Yoon S, et al. 2020. Antioxidants (Basel). 10:. PubMed
  116. Tao H, et al. 2021. Front Immunol. 12:623280. PubMed
  117. Li X, et al. 2020. Nat Commun. 4.877777778. PubMed
  118. Piao Y, et al. 2016. Neuro Oncology. 18: 1230 - 1241. PubMed
  119. Poeckel D, et al. 2009. J Biol Chem. 284:21077. PubMed
  120. Daley D, et al. 2017. Nat Med. 23:556. PubMed
  121. Luo J et al. 2018. Immunity. 49(1):107-119 . PubMed
  122. Defaye M, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:977. PubMed
  123. Ejima R, et al. 2021. Nutrients. 13:. PubMed
  124. Jiang Y, et al. 2021. Cell Death Differ. 28:3009. PubMed
  125. Wu Y, et al. 2020. Biol Reprod. 1213:102. PubMed
  126. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  127. Veremeyko T, et al. 2019. J Neurosci Res. 97:162. PubMed
  128. Fino KK, et al. 2017. Sci Rep. 5.334722222. PubMed
  129. Jokinen MP, et al. 2017. Toxicol Pathol. 45:774. PubMed
  130. Ford J, et al. 2019. Front Immunol. 2.502083333. PubMed
  131. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  132. Sasi S, et al. 2014. J Biol Chem. 289:14178. PubMed
  133. Kim D, et al. 2019. Immune Netw. 19:e32. PubMed
  134. Moon H, et al. 2019. Nat Commun. 10:2225. PubMed
  135. Levy BD, et al. 2020. J Allergy Clin Immunol. 145:335. PubMed
  136. Hosomi K, et al. 2022. Nat Commun. 13:4477. PubMed
  137. Seo SU, et al. 2021. Front Immunol. 12:697162. PubMed
  138. Burns K, et al. 2012. Endocrinology. 153:3960. PubMed
  139. Toyohara T, et al. 2020. Cell Stem Cell. 27:147. PubMed
  140. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  141. Lever JM, et al. 2019. JCI Insight. 4:e125503. PubMed
  142. Wang Y, et al. 2019. Biomed Res Int. 2019:5756189. PubMed
  143. Hong JE, et al. 2019. Front Microbiol. 0.727083333. PubMed
  144. Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed
  145. Watson N, et al. 2015. J Immunol. 194:2796. PubMed
  146. McArthur S, et al. 2020. J Clin Invest. 130:1156. PubMed
  147. Dyrna F, et al. 2018. Am J Sports Med. 46:3511. PubMed
  148. Liu J, et al. 2022. Stem Cell Res Ther. 13:247. PubMed
  149. Liu J, et al. 2022. Small. 18:e2106172. PubMed
  150. Li X, et al. 2021. Eur J Immunol. 51:2452. PubMed
  151. Yang CC, et al. 2021. Cancers (Basel). 13:. PubMed
  152. Um HN, et al. 2021. Cell Death Dis. 12:243. PubMed
  153. Thakur V, et al. 2014. Toxicol Lett. 230:322. PubMed
  154. Quilichini E, et al. 2019. Cell Mol Gastroenterol Hepatol. 0.671527778. PubMed
  155. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  156. Burger ML, et al. 2021. Cell. 184:4996. PubMed
  157. Yi X, et al. 2021. J Bone Miner Res. 36:2426. PubMed
  158. Wang Y, et al. 2021. Nat Commun. 0.570833333. PubMed
  159. Kikuchi N, et al. 2011. Am J Respir Cell Mol Biol. 45:600. PubMed
  160. Hunt LC, et al. 2021. Cell Rep. 37:109971. PubMed
  161. Nagatake T, et al. 2021. Sci Rep. 11:10426. PubMed
  162. Rios–Doria J, et al. 2017. Cancer Res. 77:2686. PubMed
  163. Zhang N, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  164. Song M, et al. 2020. Nat Commun. 11:6298. PubMed
  165. Dubik M, et al. 2021. Front Neurosci. 15:682451. PubMed
  166. Baluk P, et al. 2022. Methods Mol Biol. 2441:115. PubMed
  167. Kang SH, et al. 2018. Oncoimmunology. 8:e1515057. PubMed
  168. Ito Y, et al. 2018. J Exp Med. 215:2617. PubMed
  169. Campbell KM, et al. 2019. Cell Rep. 28:1526. PubMed
  170. Ireland L, et al. 2018. Oncogene. 293:4244. PubMed
  171. Sutiwisesak R, et al. 2020. PLoS Pathog. 16:e1009000. PubMed
RRID
AB_893504 (BioLegend Cat. No. 123101)
AB_893504 (BioLegend Cat. No. 123102)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kupffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All F4/80 Reagents Request Custom Conjugation
Description Clone Applications
Brilliant Violet 605™ anti-mouse F4/80 BM8 FC
Purified anti-mouse F4/80 BM8 FC,IHC,WB
Biotin anti-mouse F4/80 BM8 FC,IHC
FITC anti-mouse F4/80 BM8 FC
PE anti-mouse F4/80 BM8 FC
PE/Cyanine5 anti-mouse F4/80 BM8 FC
PE/Cyanine7 anti-mouse F4/80 BM8 FC
APC anti-mouse F4/80 BM8 FC
APC/Cyanine7 anti-mouse F4/80 BM8 FC
Alexa Fluor® 488 anti-mouse F4/80 BM8 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse F4/80 BM8 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse F4/80 BM8 FC
PerCP anti-mouse F4/80 BM8 FC
PerCP/Cyanine5.5 anti-mouse F4/80 BM8 FC
Alexa Fluor® 700 anti-mouse F4/80 BM8 FC
Brilliant Violet 421™ anti-mouse F4/80 BM8 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse F4/80 BM8 FC
Alexa Fluor® 594 anti-mouse F4/80 BM8 IHC-F
Brilliant Violet 785™ anti-mouse F4/80 BM8 FC
Purified anti-mouse F4/80 (Maxpar® Ready) BM8 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse F4/80 BM8 FC
Brilliant Violet 650™ anti-mouse F4/80 BM8 FC
Brilliant Violet 711™ anti-mouse F4/80 BM8 FC
APC/Fire™ 750 anti-mouse F4/80 BM8 FC
TotalSeq™-A0114 anti-mouse F4/80 BM8 PG
TotalSeq™-B0114 anti-mouse F4/80 BM8 PG
TotalSeq™-C0114 anti-mouse F4/80 BM8 PG
Spark YG™ 570 anti-mouse F4/80 BM8 IHC-F
KIRAVIA Blue 520™ anti-mouse F4/80 BM8 FC
Ultra-LEAF™ Purified anti-mouse F4/80 BM8 FC,IHC,WB
APC/Fire™ 810 anti-mouse F4/80 BM8 FC
Spark NIR™ 685 anti-mouse F4/80 BM8 FC
Spark Blue™ 550 anti-mouse F4/80 BM8 FC
Brilliant Violet 570™ anti-mouse F4/80 BM8 FC
Brilliant Violet 750™ anti-mouse F4/80 BM8 FC
PE/Fire™ 810 anti-mouse F4/80 BM8 FC
Spark Red™ 718 anti-mouse F4/80 (Flexi-Fluor™) BM8 FC
Spark Blue™ 574 anti-mouse F4/80 (Flexi-Fluor™) BM8 FC
Go To Top Version: 2    Revision Date: 10.25.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account