Purified anti-mouse F4/80 Maxpar Ready Antibody anti-F4/80

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Clone: BM8 (See other available formats)
Regulatory Status: RUO
Other Names: EMR1, Ly71
Isotype: Rat IgG2a, κ
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Product Citations: publications

Mouse bone-marrow stained with 148Nd-anti-CD11b (M1/70) and 146Nd-anti-F4/80 (BM8). Data provided by DVS Sciences.

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123143

100 µg

129 CHF

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Description

F4/80, also known as EMR1 or Ly71, is a 160 kD glycoprotein of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80 has been widely used as a murine macrophage marker. It is expressed on a majority of tissue macrophages, including macrophages in the lung, gut, peritoneal cavity, thymus, and red pulp of the spleen, Kupffer cells, Langerhans cells, microglia, and certain dendritic cells. It is not expressed on macrophages located in the T cell areas of the spleen, lymph node, or Peyer's patch. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for the induction of CD8⁺ T cells-mediated peripheral tolerance.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Murine macrophages
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^1,2 and formalin-fixed paraffin-embedded sections^6,7, Western blotting, and spatial biology (IBEX)^12,13.
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
RRID: AB_2563767 (BioLegend Cat. No. 123143)

Antigen Details

Structure: EGF-TM7 family member, 160 kD glycoprotein
Distribution: Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kupffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells
Function: Induction of immunological tolerance
Cell Type: Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References: 1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.
Gene ID: 13733 View all products for this Gene ID
UniProt: View information about F4/80 on UniProt.org

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Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All F4/80 Reagents Request Custom Conjugation

Description	Clone	Applications
Brilliant Violet 605™ anti-mouse F4/80	BM8	FC
Purified anti-mouse F4/80	BM8	FC,IHC,WB
Biotin anti-mouse F4/80	BM8	FC,IHC
FITC anti-mouse F4/80	BM8	FC
PE anti-mouse F4/80	BM8	FC
PE/Cyanine5 anti-mouse F4/80	BM8	FC
PE/Cyanine7 anti-mouse F4/80	BM8	FC
APC anti-mouse F4/80	BM8	FC
APC/Cyanine7 anti-mouse F4/80	BM8	FC
Alexa Fluor® 488 anti-mouse F4/80	BM8	FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse F4/80	BM8	FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse F4/80	BM8	FC
PerCP anti-mouse F4/80	BM8	FC
PerCP/Cyanine5.5 anti-mouse F4/80	BM8	FC
Alexa Fluor® 700 anti-mouse F4/80	BM8	FC
Brilliant Violet 421™ anti-mouse F4/80	BM8	FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse F4/80	BM8	FC
Alexa Fluor® 594 anti-mouse F4/80	BM8	IHC-F
Brilliant Violet 785™ anti-mouse F4/80	BM8	FC
Purified anti-mouse F4/80 (Maxpar® Ready)	BM8	FC,CyTOF®
PE/Dazzle™ 594 anti-mouse F4/80	BM8	FC
Brilliant Violet 650™ anti-mouse F4/80	BM8	FC
Brilliant Violet 711™ anti-mouse F4/80	BM8	FC
APC/Fire™ 750 anti-mouse F4/80	BM8	FC
TotalSeq™-A0114 anti-mouse F4/80	BM8	PG
TotalSeq™-B0114 anti-mouse F4/80	BM8	PG
TotalSeq™-C0114 anti-mouse F4/80	BM8	PG
Spark YG™ 570 anti-mouse F4/80	BM8	IHC-F
KIRAVIA Blue 520™ anti-mouse F4/80	BM8	FC
Ultra-LEAF™ Purified anti-mouse F4/80	BM8	FC,IHC,WB
APC/Fire™ 810 anti-mouse F4/80	BM8	FC
Spark NIR™ 685 anti-mouse F4/80	BM8	FC
Spark Blue™ 550 anti-mouse F4/80	BM8	FC
Brilliant Violet 570™ anti-mouse F4/80	BM8	FC
Brilliant Violet 750™ anti-mouse F4/80	BM8	FC
PE/Fire™ 810 anti-mouse F4/80	BM8	FC
Spark Red™ 718 anti-mouse F4/80 (Flexi-Fluor™)	BM8	FC
Spark Blue™ 574 anti-mouse F4/80 (Flexi-Fluor™)	BM8	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 07.21.2022

For Research Use Only. Not for diagnostic or therapeutic use.

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