Spark Red™ 718 anti-human CD107a (LAMP-1) Antibody, CD107a, H4A3

Pricing & Availability

Clone: H4A3 (See other available formats)
Regulatory Status: RUO
Workshop: P PR-63; BP 473; P P008
Other Names: Lysosome-Associated Membrane Protein 1, LGP-120, LAMP-1
Isotype: Mouse IgG1, κ
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Product Citations: publications

H4A3_SparkRed718_CD107a_Antibody_1_112124 — Thrombin-activated human peripheral blood platelets were stained with anti-human CD41/CD61 (clone A2A9/6) FITC and anti-human CD107a (LAMP-1) (clone H4A3) Spark Red™ 718 (left) or mouse IgG1, κ Spark Red™ 718 isotype control (right).

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328659

25 tests

157 CHF

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328660

100 tests

349 CHF

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Description

CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40 kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation, CD107a is transferred to the cell membrane surface of activated platelets, activated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107a has been suggested to play a role in the protection of lysosomal membrane from lysosomal hydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surface expression of LAMP-1 has been shown to correlate with CD8⁺ T cell and NK cell cytotoxicity.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human adult adherent peripheral blood cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Red™ 718 has a maximum excitation of 697 nm and a maximum emission of 718 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting⁸, immunohistochemical staining², immunofluorescence^5,7, and immunoprecipitation⁵.

This antibody is specific to human LAMP-1. Positive control: Hela cells; LAMP-1 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.

Antigen Details

Structure: LAMP-1 is a 417 amino acid protein with a molecular mass of 45 kD.
Distribution: Macrophages, epithelial cells, endothelial cells, some tumor cells; located on the luminal side of lysosomes or on the surface of cell membranes
Function: Protect lysosomal membrane from lysosomal hydrolases, adhesion
Ligand/Receptor: Galaptin
Cell Type: Endothelial cells, Epithelial cells, Macrophages
Biology Area: Cell Biology, Immunology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Sarafian V, et al. 2006. Arch. Dermatol. Res. 298:7381.
2. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press.
3. Sawada R, et al. 1993. J. Biol. Chem. 268:12675.
4. Chen JW, et al. 1988. J. Biol. Chem. 263:8754.
5. Chen JW, et al. 1986. Biochem. Soc. Symp. 51:97112.
Gene ID: 3916 View all products for this Gene ID
UniProt: View information about CD107a on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD107a Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-human CD107a (LAMP-1)	H4A3	FC
Purified anti-human CD107a (LAMP-1)	H4A3	FC,ICC,WB,IHC-P,IP,SB
FITC anti-human CD107a (LAMP-1)	H4A3	FC
PE anti-human CD107a (LAMP-1)	H4A3	FC
Alexa Fluor® 488 anti-human CD107a (LAMP-1)	H4A3	FC
Alexa Fluor® 647 anti-human CD107a (LAMP-1)	H4A3	FC
PerCP/Cyanine5.5 anti-human CD107a (LAMP-1)	H4A3	FC
APC anti-human CD107a (LAMP-1)	H4A3	FC
Pacific Blue™ anti-human CD107a (LAMP-1)	H4A3	FC
Brilliant Violet 421™ anti-human CD107a (LAMP-1)	H4A3	FC
PE/Cyanine7 anti-human CD107a (LAMP-1)	H4A3	FC
APC/Cyanine7 anti-human CD107a (LAMP-1)	H4A3	FC
Brilliant Violet 510™ anti-human CD107a (LAMP-1)	H4A3	FC
Brilliant Violet 605™ anti-human CD107a (LAMP-1)	H4A3	FC
Purified anti-human CD107a (LAMP-1) (Maxpar® Ready)	H4A3	FC,CyTOF®
Brilliant Violet 650™ anti-human CD107a (LAMP-1)	H4A3	FC
Brilliant Violet 711™ anti-human CD107a (LAMP-1)	H4A3	FC
PerCP anti-human CD107a (LAMP-1)	H4A3	FC
Brilliant Violet 785™ anti-human CD107a (LAMP-1)	H4A3	FC
PE/Dazzle™ 594 anti-human CD107a (LAMP-1)	H4A3	FC
TotalSeq™-A0155 anti-human CD107a (LAMP-1)	H4A3	PG
TotalSeq™-C0155 anti-human CD107a (LAMP-1)	H4A3	PG
TotalSeq™-B0155 anti-human CD107a (LAMP-1)	H4A3	PG
APC/Fire™ 750 anti-human CD107a (LAMP-1) Antibody	H4A3	FC
PE/Cyanine5 anti-human CD107a (LAMP-1)	H4A3	FC
Spark Blue™ 574 anti-human CD107a (LAMP-1) (Flexi-Fluor™)	H4A3	FC
Spark PLUS B550™ anti-human CD107a (LAMP-1) Antibody	H4A3	FC
Spark Red™ 718 anti-human CD107a (LAMP-1) Antibody	H4A3	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 10.23.2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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