Loading...

APC/Cyanine7 anti-mouse CD11c Antibody

Pricing & Availability
Clone
N418 (See other available formats)
Regulatory Status
RUO
Other Names
αX integrin, integrin αX chain, CR4, p150, ITGAX
Isotype
Armenian Hamster IgG
Ave. Rating
Submit a Review
Product Citations
publications
N418_APCCyanine7_CD11c_Antibody_022519
C57BL/6 mouse splenocytes were stained with I-A/I-E PE and CD11c (clone N418) APC/Cyanine7 (left) or Armenian hamster IgG APC/Cyanine7 isotype control (right).
  • N418_APCCyanine7_CD11c_Antibody_022519
    C57BL/6 mouse splenocytes were stained with I-A/I-E PE and CD11c (clone N418) APC/Cyanine7 (left) or Armenian hamster IgG APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
117323 25 µg 94 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
117324 100 µg 292 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse spleen dendritic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.

Application References

(PubMed link indicates BioLegend citation)
  1. Granucci F, et al. 1997. J. Immunol. 159:1794.
  2. Stokes RW, et al. 1998. J. Immunol. 160:5514.
  3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
  4. Ma XT, et al. 2006. Cancer Research 66:1169.
  5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
  6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
  7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
  8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
  10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
  11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
  12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
  13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
  14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
  15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
  18. Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
  19. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  20. Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
  21. White CE, et al. 2015. J Immunol. 194:697. PubMed
  22. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  23. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  24. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Sierro F, et al. 2017. Immunity. 47:374. PubMed
  2. Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed
  3. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  4. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  5. Peng J, et al. 2023. Am J Reprod Immunol. 89:e13678. PubMed
  6. Clark JT, et al. 2023. Cell Rep. 42:112147. PubMed
  7. Wang YC, et al. 2023. J Exp Med. 220:. PubMed
  8. Li G, et al. 2023. Cell Death Dis. 14:185. PubMed
  9. Zhang J, et al. 2023. Theranostics. 13:2226. PubMed
  10. Hsieh MS, et al. 2023. NPJ Vaccines. 8:82. PubMed
  11. Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed
  12. Carone M, et al. 2023. Nat Commun. 14:3489. PubMed
  13. Heindl S, et al. 2021. J Exp Med. 218:. PubMed
  14. Liu H, et al. 2022. Cancer Immunol Res. 10:1028. PubMed
  15. Mansouri S, et al. 2022. J Immunol. 209:2114. PubMed
  16. Millar FR, et al. 2022. Cell Rep. 41:111596. PubMed
  17. Zhao M, et al. 2023. J Nanobiotechnology. 21:50. PubMed
  18. Sun Y, et al. 2023. Cell Mol Life Sci. 80:63. PubMed
  19. Xiong W, et al. 2022. Nat Commun. 13:1700. PubMed
  20. Sasaki I, et al. 2012. Blood. 120:4733. PubMed
  21. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  22. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  23. Huang J, et al. 2022. J Transl Med. 20:496. PubMed
  24. Kitagawa S, et al. 2022. NPJ Vaccines. 7:115. PubMed
  25. Ortiz G, et al. 2020. Front Immunol. 11:1713. PubMed
  26. Herz J, et al. 2015. J Exp Med. 212: 1153 - 1169. PubMed
  27. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
  28. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  29. Aldon Y, et al. 2020. J Immunol. 204:903. PubMed
  30. Han Y, et al. 2020. Nat Commun. 11:1776. PubMed
  31. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  32. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  33. Deslyper G, et al. 2021. Parasitology. 148:539. PubMed
  34. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  35. Sari G, et al. 2020. Biochem Biophys Res Commun. 510:524. PubMed
  36. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  37. Yoshioka D, et al. 2016. Antimicrob Agents Chemother. 60: 6146 - 6154. PubMed
  38. Shon W, et al. 2015. Sci Rep. 5:17305. PubMed
  39. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  40. Casali P, et al. 2021. Front Immunol. 12:761450. PubMed
  41. Xu F, et al. 2022. Cell Death Discov. 8:142. PubMed
  42. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  43. Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
  44. Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed
  45. Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
  46. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  47. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  48. Kölle J, et al. 2022. iScience. 25:104440. PubMed
  49. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  50. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  51. Metzger D, et al. 2015. J Infect Dis. 212: 445-452. PubMed
  52. Dos Santos Dias L, et al. 2021. PLoS Pathog. e1009324:17. PubMed
  53. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  54. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  55. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
  56. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  57. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  58. Proto JD et al. 2018. Immunity. 49(4):666-677 . PubMed
  59. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  60. Zhang K, et al. 2014. PLoS Pathog. 10:1004385. PubMed
  61. Furuya Y, et al. 2015. PLoS Pathog. 11: 1005180. PubMed
  62. Wessel AW, et al. 2020. Nat Commun. 4.123611111. PubMed
  63. Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed
  64. Suresh MV, et al. 2019. JCI Insight. 4:e133103. PubMed
  65. Takano T, et al. 2020. Front Immunol. 11:1555. PubMed
  66. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  67. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  68. Mulas F, et al. 2020. Cell Mol Immunol. . PubMed
  69. Viana C, et al. 2015. PLoS One. 10: 0130809. PubMed
  70. Davis M, et al. 2015. J Immunol. 194:2219. PubMed
  71. Branchett WJ, et al. 2020. Wellcome Open Research. 0.278472222. PubMed
  72. Zerif E, et al. 2020. Int J Mol Sci. 21:. PubMed
  73. Ariyoshi Y, et al. 2016. Gene Ther. 10.1038/gt.2016.7. PubMed
  74. Zeng W, et al. 2021. STAR Protocols. 2(1):100361. PubMed
  75. Lin J, et al. 2021. Nat Commun. 12:5321. PubMed
  76. Galle-Treger L, et al. 2020. J Allergy Clin Immunol. 145:502. PubMed
  77. Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed
  78. Walsh SM, et al. 2021. eLife. 10:00. PubMed
  79. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  80. Kim P, et al. 2015. Infect Immun . 83: 2992 - 3002. PubMed
  81. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  82. Echevarría-Vargas IM, et al. 2018. EMBO Mol Med. 10:e8446. PubMed
  83. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  84. Zhang W, et al. 2020. Nat Commun. 11:1187. PubMed
  85. Nair S, et al. 2021. JCI Insight. 6:. PubMed
  86. Yang X, et al. 2022. Front Immunol. 13:856230. PubMed
  87. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  88. Spangenberg E, et al. 2019. Nat Commun. 10:3758. PubMed
  89. Lee H, et al. 2017. J Immunol. 199:1453. PubMed
  90. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  91. Shibuya M, et al. 2021. iScience. 24:103131. PubMed
  92. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  93. Sasaki Y, et al. 2015. PLoS One. 10: 0131176. PubMed
  94. Draijer C, et al. 2018. Sci Rep. 8:5105. PubMed
  95. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
  96. Darling R, et al. 2020. Int J Nanomedicine. 5.19375. PubMed
  97. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  98. Guendel F, et al. 2020. Immunity. 53(5):1015-1032.e8. PubMed
  99. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  100. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  101. Zhao N, et al. 2018. J Clin Invest. 26:84. PubMed
  102. Tulyeu J, et al. 2021. Curr Protoc. 1:e283. PubMed
  103. Zheng Y, et al. 2022. J Immunol. 208:501. PubMed
  104. Xiao Y, et al. 2021. Cell. 184:6037. PubMed
  105. Issah Y, et al. 2021. Elife. 10:. PubMed
  106. Konda P, et al. 2022. Am J Cancer Res. 12:210. PubMed
  107. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  108. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  109. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  110. Mogilenko DA et al. 2019. Cell. 177(5):1201-1216 . PubMed
  111. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
  112. Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
  113. Zhang L, et al. 2020. Cell. 442:181. PubMed
  114. Sheng J, et al. 2021. eLife. 10:00. PubMed
  115. Khan S, et al. 2016. J Exp Med. 213(13):2913-2929. PubMed
  116. Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed
  117. Lau A, et al. 2022. Sci Adv. 8:eabm0142. PubMed
  118. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  119. Strait AA, et al. 2021. Commun Biol. 4:1005. PubMed
  120. Lerbs T, et al. 2020. JCI Insight. 5:00. PubMed
  121. Qi S, et al. 2020. Theranostics. 10:1814. PubMed
  122. Kim I et al. 2015. Brain and behavior. 5(12):e00403 . PubMed
  123. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  124. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
  125. Zhang SJ, et al. 2021. Adv Sci (Weinh). 8:2003410. PubMed
  126. Hohsfield LA, et al. 2021. Elife. 10:. PubMed
  127. Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
  128. Choi J, et al. 2013. PLoS Negl Trop Dis. 7:e1981. PubMed
RRID
AB_830646 (BioLegend Cat. No. 117323)
AB_830646 (BioLegend Cat. No. 117324)

Antigen Details

Structure
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution

Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells

Function
Cellular adhesion
Ligand/Receptor
iC3b, fibrinogen
Cell Type
Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.

Gene ID
16411 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD11c Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD11c N418 FC
Biotin anti-mouse CD11c N418 FC,IHC-F
FITC anti-mouse CD11c N418 FC
PE anti-mouse CD11c N418 FC
Purified anti-mouse CD11c N418 FC,CyTOF®,IP,IHC
Alexa Fluor® 488 anti-mouse CD11c N418 FC
Alexa Fluor® 647 anti-mouse CD11c N418 FC,IHC,ICC,3D IHC
PE/Cyanine5 anti-mouse CD11c N418 FC
PE/Cyanine7 anti-mouse CD11c N418 FC
Brilliant Violet 605™ anti-mouse CD11c N418 FC
Alexa Fluor® 700 anti-mouse CD11c N418 FC
Pacific Blue™ anti-mouse CD11c N418 FC
APC/Cyanine7 anti-mouse CD11c N418 FC
PerCP/Cyanine5.5 anti-mouse CD11c N418 FC
PerCP anti-mouse CD11c N418 FC
Brilliant Violet 421™ anti-mouse CD11c N418 FC,IHC
Brilliant Violet 570™ anti-mouse CD11c N418 FC
Brilliant Violet 785™ anti-mouse CD11c N418 FC
Brilliant Violet 510™ anti-mouse CD11c N418 FC
Brilliant Violet 650™ anti-mouse CD11c N418 FC
Purified anti-mouse CD11c (Maxpar® Ready) N418 FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD11c N418 IHC-F,3D IHC
PE/Dazzle™ 594 anti-mouse CD11c N418 FC
Brilliant Violet 711™ anti-mouse CD11c N418 FC
APC/Fire™ 750 anti-mouse CD11c N418 FC
TotalSeq™-A0106 anti-mouse CD11c N418 PG
Brilliant Violet 750™ anti-mouse CD11c N418 FC
TotalSeq™-B0106 anti-mouse CD11c N418 PG
TotalSeq™-C0106 anti-mouse CD11c N418 PG
KIRAVIA Blue 520™ anti-mouse CD11c N418 FC
Spark Blue™ 550 anti-mouse CD11c N418 FC
Spark NIR™ 685 anti-mouse CD11c N418 FC
Spark UV™ 387 anti-mouse CD11c N418 FC
Spark Red™ 718 anti-mouse CD11c N418 FC
Spark Blue™ 515 anti-mouse CD11c N418 FC
PerCP/Fire™ 806 anti-mouse CD11c N418 FC
Spark PLUS UV395™ anti-mouse CD11c N418 FC
Spark Blue™ 574 anti-mouse CD11c (Flexi-Fluor™) N418 FC
Go To Top Version: 2    Revision Date: 02.25.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account