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PerCP/Cyanine5.5 anti-mouse CD104 Antibody

Pricing & Availability
Clone
346-11A (See other available formats)
Regulatory Status
RUO
Other Names
Integrin β4, β4 integrin, TSP-1180, ITGB4
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
346-11A_PerCPCy5.5_CD104_Antibody_061418
Mouse lung carcinoma cell line (Line 1) were stained with CD104 (clone 346-11A) PerCP/Cyanine5.5 (filled histogram) or Rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • 346-11A_PerCPCy5.5_CD104_Antibody_061418
    Mouse lung carcinoma cell line (Line 1) were stained with CD104 (clone 346-11A) PerCP/Cyanine5.5 (filled histogram) or Rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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123613 25 µg $176
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123614 100 µg $382
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Description

CD104 is a 205 kD type I transmembrane glycoprotein, also known as integrin β4 chain or β4 integrin, that associates with integrin α6 (CD49f) to form the α6/β4 (CD49f/CD104) heterodimer. CD104 is expressed on epithelial cells (especially on proliferative basal layer epithelial cells in skin), endothelial cells, Schwann cells, certain tumor cells, and a subset of pre-T cells. The CD49f/CD104 heterodimer is an adhesion receptor for laminins (especially laminin 5) and keratin filaments, and is involved in the regulation of hemidesmosome formation and cell proliferation and activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Tumor-associated antigen TSP-180 from BALB/c lung carcinoma
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. 

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining1.

Application References

(PubMed link indicates BioLegend citation)
  1. Tennenbaum T, et al. 1993. Cancer Res. 53:4803. (IHC)
Product Citations
  1. Shi X, et al. 2022. Clin Transl Med. 12:e711. PubMed
RRID
AB_2734183 (BioLegend Cat. No. 123613)
AB_2734183 (BioLegend Cat. No. 123614)

Antigen Details

Structure
Integrin β chain, 205 kD, forms heterodimer with integrin α6 (CD49f)
Distribution

Epithelial cells, endothelial cells, some tumor cells, pre-T cell subset

Function
Adhesion
Ligand/Receptor
Laminins, keratin filaments
Cell Type
Embryonic Stem Cells, Endothelial cells, Epithelial cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kennel SJ, et al. 1992. J. Cell Sciences 101:1992.

Gene ID
192897 View all products for this Gene ID
UniProt
View information about CD104 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 06/15/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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