Alexa Fluor® 488 anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
17A2_A488_CD3_Antibody_4_092121
Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse CD3 Antibody (clone 17A2) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse IgD Antibody (clone 11-26c.2a) Alexa Fluor® 594 at 5 µg/mL (blue), and anti-mouse CD68 Antibody (clone FA-11) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
Watch the video.
  • 17A2_A488_CD3_Antibody_4_092121
    Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse CD3 Antibody (clone 17A2) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse IgD Antibody (clone 11-26c.2a) Alexa Fluor® 594 at 5 µg/mL (blue), and anti-mouse CD68 Antibody (clone FA-11) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
    Watch the video.
  • 17A2_Alexa488_071805
    C57BL/6 mouse splenocytes stained with 17A2 Alexa Fluor® 488
  • 17A2_Alexa488_CD3_IHC-F_120816
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL CD3 (Clone 17A2) Alexa Fluor® 488 (green) and B220 (Clone RA3-6B2) Brilliant Violet 421™ (blue), overnight at 4°C. The image was captured by 10X objective.
  • 3_Still-image_0316-5_20X-1_1
    Formalin-fixed, 300 micron-thick mouse spleen section was blocked, permeabilized and stained overnight with CD3 (clone 17A2) Alexa Fluor® 488 (red), CD21/35 (CR2/CR1)(clone 7E9) Alexa Fluor® 594 (green), and CD45R/B220 (clone RA3-6B2) Alexa Fluor® 647 (blue) all at 5 µg/mL, optically cleared, then analyzed at 215 μm imaging depth on a confocal microscope.
Compare all formats See Alexa Fluor® 488 spectral data See high resolution IHC-F data...
Cat # Size Price Quantity Check Availability
100212 25 µg $101.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
100210 100 µg $229.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µL volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per mL. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Cautivo KM, et al. 2022. Immunity. 55:254. PubMed
  2. Jang HJ, et al. 2022. Cancer Res. 82:2593. PubMed
  3. Ariyasu Y, et al. 2022. Int J Mol Sci. 23: . PubMed
  4. Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed
  5. Liu G, et al. 2023. Commun Biol. 6:102. PubMed
  6. Vijver SV, et al. 2023. Front Immunol. 13:1100730. PubMed
  7. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  8. Feist M, et al. 2021. Cancer Gene Ther. 28:98. PubMed
  9. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  10. Axelrod ML, et al. 2022. Nature. 611:818. PubMed
  11. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
  12. Arora S, et al. 2023. PLoS One. 18:e0286211. PubMed
  13. Wu J, et al. 2022. Nat Commun. 13:7321. PubMed
  14. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
  15. Okajima T, et al. 2021. Front Immunol. 12:738041. PubMed
  16. Bieber M, et al. 2021. Int J Mol Sci. 22:. PubMed
  17. Schneider C, et al. 2018. Cell. 174:271. PubMed
  18. Dauer Née Joppe K, et al. 2021. J Neurochem. 159:554. PubMed
  19. Datta S, et al. 2014. J Leukoc Biol. 95:853. PubMed
  20. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  21. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  22. Park JJ, et al. 2021. Nat Commun. 12:1222. PubMed
  23. Upadhyaya P, et al. 2021. J Immunother Cancer. 9:00. PubMed
  24. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  25. Kodumudi KN, et al. 2019. Front Immunol. 10:1939. PubMed
  26. Logan C, Bowen C, and Menko A 2017. Sci Rep. . 10.1038/s41598-017-16456-5. PubMed
  27. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  28. Lau CH, et al. 2022. Antioxidants (Basel). 11:. PubMed
  29. Wang C, et al. 2021. Cell Stem Cell. . PubMed
  30. Wang H et al. 2017. Developmental cell. 40(6):566-582 . PubMed
  31. Mills C, et al. 2022. Cells. 11:. PubMed
  32. Wan W, et al. 2013. Cardiovasc Res. 97:580. PubMed
  33. Wan W, et al. 2015. Cardiovasc Res. 106:478. PubMed
  34. Amor C, et al. 2020. Nature. 583:127. PubMed
  35. Wei SC, et al. 2020. Cancer Discov. . PubMed
  36. Wong HS, et al. 2021. Cell. . PubMed
  37. Xiao S, et al. 2007. J Exp Med. 204:1691. PubMed
  38. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  39. Hsieh C, et al. 2014. J Immunol. 193:3693. PubMed
  40. Counoupas C, et al. 2020. NPJ Vaccines. 0.28125. PubMed
  41. Wang B, et al. 2021. J Immunother Cancer. 9:. PubMed
  42. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
  43. Pietronigro E, et al. 2019. Sci Rep. 9:12055. PubMed
  44. Riding AM, et al. 2022. iScience. 25:104660. PubMed
  45. Hanihara-Tatsuzawa F, et al. 2014. J Biol Chem. 389:30925. PubMed
  46. Broderick L, et al. 2019. Nat Commun. 10:3644. PubMed
  47. Karandikar SH, et al. 2019. Anal Chem. 91:3405. PubMed
  48. Langhauser F, et al. 2014. Stroke. 45:1799. PubMed
  49. Karandikar SH, et al. 2019. JCI Insight. 5. PubMed
  50. Wang S, et al. 2022. J Inflamm Res. 14:7107. PubMed
  51. Xu JL, et al. 2022. Front Pharmacol. 13:911663. PubMed
  52. Li MY, et al. 2021. Developmental Cell. 56:2547. PubMed
  53. MaruYama T, et al. 2015. J Leukoc Biol. 98: 385-393. PubMed
  54. Cheng Q, et al. 2018. PLoS Pathog. 14:e1007440. PubMed
  55. Liu Y, et al. 2019. Sci Rep. 9:18970. PubMed
  56. Zhao L,et al. 2017. Nat Commun. . 10.1038/s41467-017-01181-4. PubMed
  57. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  58. Zhou P, et al. 2022. Mol Ther Oncolytics. 25:236. PubMed
  59. Thurlow LR, et al. 2018. Cell Host Microbe. 24:261. PubMed
  60. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
  61. Lee-Sayer SSM, et al. 2018. Eur J Immunol. 48:803. PubMed
  62. Hsing CH, et al. 2021. Biomedicines. 9:. PubMed
  63. Kuhn NF et al. 2019. Cancer cell. 35(3):473-488 . PubMed
RRID
AB_389301 (BioLegend Cat. No. 100212)
AB_389301 (BioLegend Cat. No. 100210)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC,IHC-F,3D IHC,SB
Alexa Fluor® 488 anti-mouse CD3 17A2 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC,ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Spark Red™ 718 anti-mouse CD3 17A2 FC
Spark UV™ 387 anti-mouse CD3 17A2 FC
Go To Top Version: 5    Revision Date: 09/21/2021

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account