Alexa Fluor® 488 anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
HK1dot4_AF488_071510
C57BL/6 bone marrow cells stained with anti-mouse Ly-6C, HK1.4 Alexa Fluor® 488
  • HK1dot4_AF488_071510
    C57BL/6 bone marrow cells stained with anti-mouse Ly-6C, HK1.4 Alexa Fluor® 488
Compare all formats See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Check Availability
128021 25 µg $124.00
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128022 100 µg $265.00
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
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  4. Baratchart E, et al. 2022. PLoS Comput Biol. 18:e1009839. PubMed
  5. Sundarasivarao PYK, et al. 2022. Front Immunol. 13:1011944. PubMed
  6. Draijer C, et al. 2023. Front Immunol. 13:1056477. PubMed
  7. Pinto NA, et al. 2023. Cell Death Differ. :. PubMed
  8. Yang L, et al. 2023. Sci Rep. 13:1827. PubMed
  9. Zander R, et al. 2022. Immunity. 55:475. PubMed
  10. Karlen SJ, et al. 2018. J Neuroinflammation. 15:344. PubMed
  11. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  12. Vaibhav K, et al. 2018. J Exp Med. 215:2636. PubMed
  13. Alexander J, et al. 2012. Kidney Int. 82:961. PubMed
  14. Lu X, et al. 2020. Sci Transl Med. 12:. PubMed
  15. Guo P, et al. 2021. J Immunol. 207:408. PubMed
  16. Zenker S, et al. 2014. J Immunol. 192:2830. PubMed
  17. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  18. Zhou Z, et al. 2022. Life (Basel). 12:. PubMed
  19. , et al. 2021. Eur J Immunol. 51:2708. PubMed
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  21. Dang MT, et al. 2021. Cell Reports. 34(13):108917. PubMed
  22. Toomey CB, et al. 2018. Invest Ophthalmol Vis Sci. 59:662. PubMed
  23. Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
  24. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  25. Toomey C, et al. 2015. Proc Natl Acad Sci U S A. 112:3040. PubMed
  26. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  27. Geng T, et al. 2021. J Infect Dis. 223:2186. PubMed
  28. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  29. Pizzurro GA, et al. 2021. Cancers (Basel). 13:. PubMed
  30. Lo CH, et al. 2021. Sci Rep. 6055:11. PubMed
  31. Breznik JA, et al. 2021. The Journal of Physiology. 599(8):2169-2195. PubMed
  32. Yousef H, et al. 2019. Nat Med. 25:988. PubMed
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  35. Skokowa J, et al. 2022. Nat Commun. 13:2948. PubMed
  36. Kelley WJ, et al. 2022. JCI Insight. 7: . PubMed
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  38. Fujimura N, et al. 2016. Sci Rep. 5:11664. PubMed
  39. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
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  41. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  42. Ishihara J, et al. 2018. Nat Commun. 9:2163. PubMed
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RRID
AB_10640820 (BioLegend Cat. No. 128021)
AB_10640820 (BioLegend Cat. No. 128022)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org
Go To Top Version: 3    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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