Pacific Blue™ anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
HK1dot4_Blue_080709.jpg
C57BL/6 mouse bone marrow cells stained with HK1.4 Pacific Blue™
  • HK1dot4_Blue_080709.jpg
    C57BL/6 mouse bone marrow cells stained with HK1.4 Pacific Blue™
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability
128013 25 µg $124.00
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128014 100 µg $265.00
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Frieler RA, et al. 2017. Exp Neurol. 298:104. PubMed
  2. Mathur R, et al. 2019. Mucosal Immunol. 12:612. PubMed
  3. Cheng MI, et al. 2023. Nat Immunol. 24:780. PubMed
  4. Yang C, et al. 2022. Nat Commun. 13:3426. PubMed
  5. Song R, et al. 2022. Immunology. 165:274. PubMed
  6. Lee DE, et al. 2022. Nat Commun. 13:7613. PubMed
  7. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  8. Orberg E, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.53. PubMed
  9. Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed
  10. Zhang R, et al. 2019. Cell Rep. 28:2647. PubMed
  11. Maschmeyer P, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.005. PubMed
  12. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  13. Jenull S, et al. 2021. Cell Reports. 36(3):109406. PubMed
  14. Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
  15. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  16. Bourn JR, et al. 2021. Cancer Lett. 503:75. PubMed
  17. Lebratti T, et al. 2021. eLife. 10:00. PubMed
  18. Spees A, et al. 2014. Infect Immun . 82:1692. PubMed
  19. Li J, et al. 2019. JCI Insight. 5. PubMed
  20. Mooney J, et al. 2015. Sci Rep. 5: 14603. PubMed
  21. Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed
  22. Kim S, et al. 2022. Redox Biol. 54:102347. PubMed
  23. Chung L et al. 2018. Cell host & microbe. 23(2):203-214 . PubMed
  24. Hoffmann J, et al. 2014. PLoS One. 9:98456. PubMed
  25. Jin C, et al. 2019. Cell. 176:998. PubMed
  26. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  27. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  28. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  29. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  30. Bitschar K, et al. 2019. Nat Commun. 10:2730. PubMed
  31. Cunha LD et al. 2018. Cell. 175(2):429-441 . PubMed
  32. Li S, et al. 2019. Front Neurol. 1.036805556. PubMed
  33. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  34. Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed
  35. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
  36. Muchenditsi A, et al. 2017. Am J Physiol Gastrointest Liver Physiol. 313:G39. PubMed
  37. Sadhu S, et al. 2021. J Immunol. 207:1812. PubMed
  38. Graham JB, et al. 2017. Curr Protoc Mouse Biol. 7:221. PubMed
  39. Manríquez V, et al. 2021. Nat Commun. 12:4547. PubMed
  40. Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
  41. Gharib S, et al. 2014. J Leukoc Biol. 95:9. PubMed
  42. Kusnadi A, et al. 2020. Immunity. 51(2):241-257. PubMed
  43. Ho A, et al. 2011. J Immunol. 187:6011. PubMed
  44. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
RRID
AB_1732079 (BioLegend Cat. No. 128013)
AB_1732079 (BioLegend Cat. No. 128014)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org
Go To Top Version: 2    Revision Date: 12/09/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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