Purified anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
HK1dot4_Pure_070808.jpg
C57BL/6 mouse bone marrow cells stained with purified HK1.4, followed by anti-rat IgG PE (gated on myeloid cell population)
  • HK1dot4_Pure_070808.jpg
    C57BL/6 mouse bone marrow cells stained with purified HK1.4, followed by anti-rat IgG PE (gated on myeloid cell population)
  • HK1dot4_Pure_Ly6c_Antibody_2_102518
    Fresh, frozen mouse spleen was stained with purified Ly6C clone HK1.4 conjugated and detected with a Cy5 CODEX™ oligonucleotide duplex (red). Samples were counterstained with TCR FITC (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
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128001 50 µg $65.00
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128002 500 µg $235.00
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Filippini D, et al. 2019. Sci Rep. 9:12286. PubMed
  2. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  3. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  4. Li F, et al. 2020. EMBO J. 39:e103205. PubMed
  5. Liu CJ, et al. 2020. Neuro Oncol. 22:1276. PubMed
  6. Blériot C, et al. 2020. Methods Mol Biol. 2164:87. PubMed
  7. Cai S, et al. 2020. Sci Rep. 10:14249. PubMed
  8. Calcagno DM, et al. 2020. Sci Immunol. 5: . PubMed
  9. Valet C, et al. 2022. J Clin Invest. 132: . PubMed
  10. Campisi L, et al. 2022. Nature. 606:945. PubMed
  11. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  12. Hezaveh K, et al. 2022. Immunity. 55:324. PubMed
  13. Potempa M, et al. 2022. J Immunol. 208:1742. PubMed
  14. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  15. Mazor R, et al. 2018. Proc Natl Acad Sci U S A. 115:E733. PubMed
  16. Bajaña S, et al. 2021. Front Immunol. 11:577718. PubMed
  17. Nobs SP, et al. 2021. J Allergy Clin Immunol. . PubMed
  18. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  19. Reglero-Real N, et al. 2021. Immunity. :. PubMed
  20. Karhadkar TR, et al. 2021. J Pharmacol Exp Ther. 376:106. PubMed
  21. Ma C, et al. 2018. Science. 360:eaan5931. PubMed
  22. Stefanescu C, et al. 2021. Front Oncol. 11:765151. PubMed
  23. Orecchioni M, et al. 2022. Methods Mol Biol. 2419:779. PubMed
  24. Wang Y, et al. 2021. Cancer Cell. 39:1375. PubMed
  25. Jackson JW, et al. 2021. Mol Ther Oncolytics. 22:444. PubMed
  26. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  27. Silvestre-Roig C, et al. 2019. Nature. 569:236. PubMed
  28. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  29. O'Dea KP, et al. 2020. J Extracell Vesicles. 9:1706708. PubMed
  30. Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
  31. Yan J, et al. 2020. Cancer Immunol Res. 8:356. PubMed
  32. Bertino P, et al. 2019. Oncoimmunology. 8:1601482. PubMed
  33. Seelige R, et al. 2018. Sci Rep. 8:13670. PubMed
  34. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  35. Hofstee MI, et al. 2021. Eur Cell Mater. 41:774. PubMed
  36. Singh PP, et al. 2021. Ocul Surf. 21:271. PubMed
  37. Oba T, et al. 2021. J Immunother Cancer. 9:. PubMed
  38. Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
  39. Lai JF, et al. 2020. J Allergy Clin Immunol. 146:1406. PubMed
  40. Francis M, et al. 2017. Toxicol Sci. 155(1):182-195. PubMed
  41. Kapellos TS, et al. 2017. Mediators Inflamm. 2017:4315412. PubMed
  42. Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed
  43. Hu G, et al. 2021. Nat Commun. 12:773. PubMed
  44. Enders M, et al. 2020. J Immunol. 204:87. PubMed
  45. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  46. Davies CL, et al. 2019. Front Immunol. 10:1048. PubMed
  47. Majer O, et al. 2019. Nature. 575:366. PubMed
  48. Lim CS, et al. 2020. Front Immunol. 11:1186. PubMed
  49. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  50. Zander R, et al. 2017. Cell Rep.. 10.1016/j.celrep.2017.10.077. PubMed
  51. Blriot C, et al. 2021. Immunity. :. PubMed
  52. Fino KK, et al. 2017. Sci Rep. 5.334722222. PubMed
  53. Ford J, et al. 2019. Front Immunol. 2.502083333. PubMed
  54. Mensurado S, et al. 2018. PLoS Biol. 16:e2004990. PubMed
  55. Karmaus PWF, et al. 2019. Nature. 565:101. PubMed
  56. Li J, et al. 2021. Front Immunol. 12:710406. PubMed
  57. Kim D, et al. 2019. Immune Netw. 19:e32. PubMed
  58. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  59. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  60. Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed
  61. Rogers P, Driessnack M, and Schwartz E. 2017. PLoS One. 10.1371/journal.pone.0181985. PubMed
  62. Qiu Z, et al. 2022. Cancer Discov. 12:502. PubMed
  63. Watson N, et al. 2015. J Immunol. 194:2796. PubMed
  64. Goltsev Y et al. 2018. Cell. 174(4):968-981 . PubMed
  65. Cané S, et al. 2021. J Immunother Cancer. 9:. PubMed
  66. Udumula MP, et al. 2021. Mol Metab. 53:101272. PubMed
  67. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  68. Zhang C, et al. 2020. Hear Res. 393:107996. PubMed
  69. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  70. An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed
  71. Kikuchi K, et al. 2018. J Immunol. 201:635. PubMed
  72. Hofstee MI, et al. 2021. Pathogens. 10:. PubMed
  73. Jones GS, et al. 2020. mSphere. 5:. PubMed
  74. Song M, et al. 2020. Nat Commun. 11:6298. PubMed
  75. De Alessandris S, et al. 2019. J Leukoc Biol. 105:1183. PubMed
  76. Wichaiyo S, et al. 2019. Haematologica. 104:1648. PubMed
  77. Sun D, et al. 2020. PLoS Pathog. 16:e1008361. PubMed
RRID
AB_1134213 (BioLegend Cat. No. 128001)
AB_1134213 (BioLegend Cat. No. 128002)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org
Go To Top Version: 3    Revision Date: 10/25/2018

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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