Biotin anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
HK1dot4_Biotin_082808.jpg
BALB/c bone marrow cells stained with HK1.4 biotin, followed by Sav-PE
  • HK1dot4_Biotin_082808.jpg
    BALB/c bone marrow cells stained with HK1.4 biotin, followed by Sav-PE
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Cat # Size Price Quantity Check Availability
128003 50 µg $77.00
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128004 500 µg $276.00
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Slusarczyk P, et al. 2023. Elife. 12: . PubMed
  2. Westermark L, et al. 2014. Infect Immun . 82:1181. PubMed
  3. Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
  4. Periasamy S, et al. 2016. PLoS Pathog. 12: 1005517. PubMed
  5. Monnerat G, et al. 2016. Nat Commun. 7:13344. PubMed
  6. O'Dea KP, et al. 2020. J Extracell Vesicles. 9:1706708. PubMed
  7. Funk KE, et al. 2019. J Neuroinflammation. 16:22. PubMed
  8. D'Agostino MR, et al. 2022. STAR Protoc. 3:101652. PubMed
  9. Oswald DM, et al. 2020. Glycoconj J. 37:395. PubMed
  10. Yao Y, et al. 2018. Cell. 175:1634. PubMed
  11. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  12. Mohammadpour H, et al. 2021. Cell Rep. 37:109883. PubMed
  13. Celias DP, et al. 2022. STAR Protoc. 3:101464. PubMed
RRID
AB_1236552 (BioLegend Cat. No. 128003)
AB_1236552 (BioLegend Cat. No. 128004)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org
Go To Top Version: 2    Revision Date: 12/09/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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