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Alexa Fluor® 700 anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
17A2_Alx700_021808
C57BL/6 mouse splenocytes stained with 17A2 Alexa Fluor® 700
  • 17A2_Alx700_021808
    C57BL/6 mouse splenocytes stained with 17A2 Alexa Fluor® 700
Compare all formats See Alexa Fluor® 700 spectral data
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100215 25 µg $94
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100216 100 µg $215
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  2. Ozga AJ, et al. 2022. Immunity. 55:82. PubMed
  3. Chae CS, et al. 2022. Cancer Discov. 12:1904. PubMed
  4. Steudler J, et al. 2022. Glia. 70:2045. PubMed
  5. Gary EN, et al. 2022. Cell Rep Med. 3:100693. PubMed
  6. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  7. Briukhovetska D, et al. 2023. Immunity. 56:143. PubMed
  8. Feng J, et al. 2022. Immunity. 55:405. PubMed
  9. Ou W, et al. 2023. Nat Commun. 14:392. PubMed
  10. Ke F, et al. 2023. Elife. 12:. PubMed
  11. Saha S, et al. 2023. Sci Rep. 13:4609. PubMed
  12. Cignarella F et al. 2018. Cell metabolism. 27(6):1222-1235 . PubMed
  13. Kwong Chung CK, et al. 2017. Immunology. 150:221. PubMed
  14. Abdul-Careem MF, et al. 2012. PLoS One. 7:e32821. PubMed
  15. Groenen AG, et al. 2022. J Lipid Res. 63:100167. PubMed
  16. Fisher E, et al. 2021. Front Immunol. 11:602254. PubMed
  17. Wojno E, et al. 2011. J Immunol. 187:266. PubMed
  18. Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed
  19. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  20. Jiang W, et al. 2022. Front Immunol. 13:878832. PubMed
  21. Toomer G, et al. 2022. Viruses. 14:. PubMed
  22. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  23. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  24. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  25. Ma YV, et al. 2021. MAbs. 13:2003281. PubMed
  26. Teng ZX, et al. 2022. J Inflamm Res. 15:3187. PubMed
  27. Yu M, et al. 2021. J Clin Invest. 131:. PubMed
  28. Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
  29. Zhang C, et al. 2020. Cell Metabolism. 31(1):148-161.e5.. PubMed
  30. Zhang H, et al. 2020. Cancer Cell. 37(1):37-54.e9.. PubMed
  31. Gary EN, et al. 2021. iScience. 24(7):102699. PubMed
  32. Jiang H, et al. 2021. Oncoimmunology. 10:1943180. PubMed
  33. Willingham SB, et al. 2018. Cancer Immunol Res. 6:1136. PubMed
  34. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  35. Levescot A, et al. 2021. J Clin Invest. 131:. PubMed
  36. Leonard JD et al. 2017. Immunity. 47(1):107-117 . PubMed
  37. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  38. Watanabe K, et al. 2018. JCI Insight. 3. PubMed
  39. Everard A, et al. 2014. Nat Commun. 5:5648. PubMed
  40. Tyagi AM, et al. 2021. eLife. 10:00. PubMed
  41. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  42. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  43. Gary E, et al. 2020. Vaccine. 3821:38. PubMed
  44. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  45. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  46. Reese B, et al. 2020. J Immunol. 205:3218. PubMed
  47. La Rose AM, et al. 2021. Mol Metab. 53:101265. PubMed
  48. Li Q, et al. 2019. Front Pharmacol. 10:786. PubMed
  49. Sharma M, et al. 2020. Circ Res. 127:335. PubMed
  50. Li A, et al. 2020. J Virol. 94:00:00. PubMed
  51. Kuhn JA, et al. 2021. Elife. 10:. PubMed
  52. Green WD, et al. 2022. J Leukoc Biol. 111:147. PubMed
  53. Kaur A, et al. 2019. Cancer Discov. 9:64. PubMed
  54. Kiniry BE, et al. 2018. J Immunol. 200:1876. PubMed
  55. Liu S, et al. 2020. Cell Host & Microbe. 26(6):779-794.e8.. PubMed
  56. Wu S, et al. 2022. BMC Immunol. 23:17. PubMed
  57. Mantilla-Rojas C, et al. 2022. Cancers (Basel). 14:. PubMed
  58. Bendtsen SK, et al. 2022. Oncoimmunology. 11:2026020. PubMed
  59. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  60. Wuggenig P, et al. 2020. Commun Biol. 3:130. PubMed
  61. Ryan NM, et al. 2022. Front Immunol. 13:932742. PubMed
  62. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  63. Bruder J, et al. 2017. Mol Ther Methods Clin Dev. 10.1016/j.omtm.2017.08.003. PubMed
  64. Echevarría-Vargas IM, et al. 2018. EMBO Mol Med. 10:e8446. PubMed
  65. Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
  66. Moguche AO et al. 2017. Cell host & microbe. 21(6):695-706 . PubMed
  67. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  68. Dulken BW, et al. 2019. Nature. 571:205. PubMed
  69. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
  70. Rosenbaum SR, et al. 2020. Cell Rep. 30:510. PubMed
  71. Godwin MS, et al. 2019. JCI Insight. 4:e126070. PubMed
  72. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  73. Agashe VV, et al. 2018. J Immunol. 201:772. PubMed
  74. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  75. Tulyeu J, et al. 2021. Curr Protoc. 1:e283. PubMed
  76. Godwin MS, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 320:L393. PubMed
  77. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  78. Muchenditsi A, et al. 2017. Am J Physiol Gastrointest Liver Physiol. 313:G39. PubMed
  79. Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed
  80. Zaitseva M, et al. 2017. Antiviral Research. 10.1016/j.antiviral.2017.05.002. PubMed
  81. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  82. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  83. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
  84. Lang JF et al. 2019. Nat Commun. 10(1):3415 . PubMed
  85. Voeller J, et al. 2019. J Immunother Cancer. 0.530555556. PubMed
  86. Mykkänen O, et al. 2014. PLoS One. 9:114790. PubMed
  87. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
  88. Schulze J, et al. 2021. Stroke. 52:2939. PubMed
  89. Spencer AJ, et al. 2022. EBioMedicine. 77:103902. PubMed
  90. Chavez JS, et al. 2022. Cells. 11:. PubMed
  91. Platteel ACM, et al. 2018. Front Immunol. 1.544444444. PubMed
  92. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  93. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  94. Sharma M, et al. 2019. Immunometabolism. 1. PubMed
  95. Kong IY, et al. 2020. Cell Rep. 33:108290. PubMed
  96. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  97. Parigger T, et al. 2021. Int J Mol Sci. 22:. PubMed
  98. Hendin HE, et al. 2022. NPJ Vaccines. 7:42. PubMed
  99. Hebbandi Nanjundappa R, et al. 2017. Cell. 171:655. PubMed
  100. Rezende RM, et al. 2019. J Immunol. 203:2621. PubMed
  101. Ye Y, et al. 2020. Genome Med. 0.557638889. PubMed
  102. Samuelson DR, et al. 2021. Commun Biol. 4:997. PubMed
  103. Jiang W, et al. 2021. Cell Rep. 37:110112. PubMed
  104. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
  105. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
RRID
AB_493696 (BioLegend Cat. No. 100215)
AB_493696 (BioLegend Cat. No. 100216)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC,IHC-F,3D IHC,SB
Alexa Fluor® 488 anti-mouse CD3 17A2 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC,ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Spark Red™ 718 anti-mouse CD3 17A2 FC
Spark UV™ 387 anti-mouse CD3 17A2 FC
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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